Low-dose agrochemicals and lawn-care pesticides induce developmental toxicity in murine preimplantation embryos.
Low-dose agrochemicals and lawn-care pesticides induce developmental toxicity in murine preimplantation embryos.
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DOI：
10.1289/ehp.6774
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发表时间：
2004-05
影响因子：
10.4
通讯作者：
Berg RL
中科院分区：
文献类型：
--
作者：
Greenlee AR;Ellis TM;Berg RL
职业接触农药可能会增加父母不孕和不良妊娠结局的风险，例如自然流产、早产和先天性异常。人们对农药的住宅使用及其对繁殖和发育造成的风险知之甚少。在本研究中，我们评估了与环境相关的低剂量接触农用化学品和草坪护理杀虫剂对小鼠植入前胚胎发育（相当于人类受孕后前 5-7 天）的直接影响。测试的药剂为美国中西部北部常用的药剂，包括六种除草剂[莠去津、麦草畏、异丙甲草胺、2,4-二氯苯氧乙酸 (2,4-D)]、二甲戊乐灵和甲草灵）、三种杀虫剂（毒死蜱、特丁磷和氯菊酯）、两种杀菌剂（百菌清和代森锰锌）、一种干燥剂（敌草快）和一种肥料（硝酸铵）。将 20-25 个胚胎组成的组与单独的化学品或化学品混合物一起体外培养 96 小时，模拟处理农药、吸入漂移物或摄入受污染地下水时遇到的暴露情况。用单独的农药孵化胚胎会增加 13 种化学物质中的 11 种的细胞凋亡（细胞死亡）百分比（p <或= 0.05），并减少 13 种化学物质中的 3 种的胚泡发育和每个胚胎的平均细胞数（p <或= 0.05）。模拟芽前除草剂、芽后除草剂和杀菌剂的混合物增加了暴露胚胎中的细胞凋亡百分比（p <或= 0.05）。模拟地下水污染物、杀虫剂配方和草坪护理除草剂的混合物会减少囊胚的发育和每个胚胎的平均细胞数（p <或= 0.05）。我们的数据表明，农药引起的伤害可能发生在发育的早期，使用多种药剂，并且浓度假定不会对人类健康造成不良影响。
Occupational exposures to pesticides may increase parental risk of infertility and adverse pregnancy outcomes such as spontaneous abortion, preterm delivery, and congenital anomalies. Less is known about residential use of pesticides and the risks they pose to reproduction and development. In the present study we evaluate environmentally relevant, low-dose exposures to agrochemicals and lawn-care pesticides for their direct effects on mouse preimplantation embryo development, a period corresponding to the first 5-7 days after human conception. Agents tested were those commonly used in the upper midwestern United States, including six herbicides [atrazine, dicamba, metolachlor, 2,4-dichlorophenoxyacetic acid (2,4-D)], pendimethalin, and mecoprop), three insecticides (chlorpyrifos, terbufos, and permethrin), two fungicides (chlorothalonil and mancozeb), a desiccant (diquat), and a fertilizer (ammonium nitrate). Groups of 20-25 embryos were incubated 96 hr in vitro with either individual chemicals or mixtures of chemicals simulating exposures encountered by handling pesticides, inhaling drift, or ingesting contaminated groundwater. Incubating embryos with individual pesticides increased the percentage of apoptosis (cell death) for 11 of 13 chemicals (p <or= 0.05) and reduced development to blastocyst and mean cell number per embryo for 3 of 13 agents (p <or= 0.05). Mixtures simulating preemergent herbicides, postemergent herbicides, and fungicides increased the percentage of apoptosis in exposed embryos (p <or= 0.05). Mixtures simulating groundwater contaminants, insecticide formulation, and lawn-care herbicides reduced development to blastocyst and mean cell number per embryo (p <or= 0.05). Our data demonstrate that pesticide-induced injury can occur very early in development, with a variety of agents, and at concentrations assumed to be without adverse health consequences for humans.
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影响因子：
5
作者：
Bell, EM;Hertz-Picciotto, I;Beaumont, JJ
通讯作者：
Beaumont, JJ
影响因子：
10.4
作者：
Garry, VF;Schreinemachers, D;Griffith, J
通讯作者：
Griffith, J
影响因子：
3.3
作者：
Amstislavksy, SY;Kizilova, EA;Eroschenko, VP
通讯作者：
Eroschenko, VP
居住在美国明尼苏达州红河谷的农药施用者出生的儿童出生缺陷，季节和性别。
影响因子：
10.4
作者：
Garry, Vincent F;Harkins, Mary E;Erickson, Leanna L;Long-Simpson, Leslie K;Holland, Seth E;Burroughs, Barbara L
通讯作者：
Burroughs, Barbara L
影响因子：
10.4
作者：
Foster, WG
通讯作者：
Foster, WG