Function and interaction of the coupled genes responsible for Pik-h encoded rice blast resistance.
Function and interaction of the coupled genes responsible for Pik-h encoded rice blast resistance.
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Pik-h编码稻瘟病抗性耦合基因的功能和相互作用
DOI：
10.1371/journal.pone.0098067
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发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Pan Q
中科院分区：
文献类型：
--
作者：
Zhai C;Zhang Y;Yao N;Lin F;Liu Z;Dong Z;Wang L;Pan Q
Pik的等位基因Pik-h赋予对稻瘟病病原菌Magnaporthe potassium的抗性。其定位克隆显示其包含一对NBS-LRR基因Pikh-1和Pikh-2。虽然Pikh-1似乎是组成型转录的，但Pikh-2的转录物丰度对病原体的挑战做出响应。Pikh-1 CC（卷曲螺旋）结构域直接与AvrPik-h和Pikh-2相互作用。瞬时表达分析表明，Pikh-2介导宿主防御反应的启动。Pikh-1和Pikh-2的核质分配是其功能所必需的。在提出的Pik-h抗性的机制模型中，建议Pikh-1充当AvrPik-h和Pikh-2之间的适配器，而Pikh-2转导信号以触发Pik-h特异性抗性。
Pik-h, an allele of Pik, confers resistance against the rice blast pathogen Magnaporthe oryzae. Its positional cloning has shown that it comprises a pair of NBS-LRR genes, Pikh-1 and Pikh-2. While Pikh-1 appears to be constitutively transcribed, the transcript abundance of Pikh-2 responds to pathogen challenge. The Pikh-1 CC (coiled coil) domain interacts directly with both AvrPik-h and Pikh-2. Transient expression assays demonstrated that Pikh-2 mediates the initiation of the host defence response. Nucleocytoplasmic partitioning of both Pikh-1 and Pikh-2 is required for their functionalities. In a proposed mechanistic model of Pik-h resistance, it is suggested that Pikh-1 acts as an adaptor between AvrPik-h and Pikh-2, while Pikh-2 transduces the signal to trigger Pik-h-specific resistance.
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影响因子：
64.5
作者：
Caplan, Jeffrey L.;Mamillapalli, Padmavathi;Dinesh-Kumar, S. P.
通讯作者：
Dinesh-Kumar, S. P.
影响因子：
4.9
作者：
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影响因子：
11.4
作者：
Jia, Y;McAdams, SA;Valent, B
通讯作者：
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影响因子：
9.8
作者：
Burch-Smith TM;Schiff M;Caplan JL;Tsao J;Czymmek K;Dinesh-Kumar SP
通讯作者：
Dinesh-Kumar SP
影响因子：
9.5
作者：
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通讯作者：
Dangl JL