Sequence homology between cloned caprine arthritis encephalitis virus and visna virus, two neurotropic lentiviruses
Sequence homology between cloned caprine arthritis encephalitis virus and visna virus, two neurotropic lentiviruses
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克隆的山羊关节炎脑炎病毒和维斯纳病毒（两种嗜神经慢病毒）之间的序列同源性
作者：
J. Pyper;J. Clements;M. Gonda;O. Narayan
山羊关节炎脑炎病毒（CAEV）是一种外源性非致癌逆转录病毒，可引起山羊神经系统疾病和致残性关节炎。从未整合的病毒DNA中克隆了长度分别为9.4和0.4个内切酶的两个片段的完整CAEV基因组。这些克隆的生物活性通过连接片段，然后转染到山羊滑膜细胞上来测试;回收感染性病毒。用异源双链体和分子杂交技术对克隆的CAEV和绵羊亲神经性病毒维斯纳病毒进行了比较。这些数据表明，最大的整体保守的核苷酸序列发生在gag和pol基因区域和两个较小的区域，SOR和推定的达特基因。最大分歧的区域发生在env基因中，特别是主要定位在编码糖基化外膜蛋白的区域中。这些发现和最近证明的遗传关系维斯纳病毒，CAEV，和人类T细胞嗜淋巴细胞病毒III型，获得性免疫缺陷综合征的病原体，可能有重要的意义，这些相关病毒的生物学特性的人类和兽医。
Caprine arthritis encephalitis virus (CAEV) is an exogenous, nononcogenic retrovirus which causes neurological disease and crippling arthritis in goats. A complete CAEV genome was cloned from unintegrated viral DNA in two fragments of 9.4 and 0.4 kilobases in length, respectively. The biological activity of these clones was tested by ligation of the fragments followed by transfection onto goat synovial membrane cells; infectious virus was recovered. Cloned CAEV and visna virus, a related neurotropic virus of sheep, were compared by heteroduplex and molecular hybridization analyses. These data demonstrated that the greatest overall conservation of nucleotide sequences occurred in the gag and pol gene regions and two smaller regions, sor and the putative tat gene. The region of greatest divergence occurred in the env gene and, in particular, was localized primarily in the region coding for the glycosylated outer membrane protein. These findings and the recently demonstrated genetic relationship of visna virus, CAEV, and human T-cell lymphotropic virus type III, the etiologic agent of the acquired immune deficiency syndrome, may have important implications concerning the biological properties of these related viruses for human and veterinary medicine.
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影响因子：
56.9
作者：
GONDA, MA;WONGSTAAL, F;GILDEN, RV
通讯作者：
GILDEN, RV
山羊关节炎脑炎病毒、维斯纳病毒和进行性肺炎病毒之间的抗原交叉反应涉及所有病毒体相关蛋白和糖蛋白。
DOI：
10.1099/0022-1317-66-6-1233
发表时间：
1985
期刊：
The Journal of general virology
影响因子：
--
作者：
Gogolewski,RP;Adams,DS;McGuire,TC;Banks,KL;Cheevers,WP
通讯作者：
Cheevers,WP
维斯纳病毒的顺式和反式转录调控。
DOI：
10.1126/science.2990051
发表时间：
1985
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Hess,JL;Clements,JE;Narayan,O
通讯作者：
Narayan,O
人类 T 细胞嗜淋巴细胞病毒 III 型基因组上反式激活区的位置。
DOI：
10.1126/science.2990041
发表时间：
1985
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Sodroski,J;Patarca,R;Rosen,C;Wong-Staal,F;Haseltine,W
通讯作者：
Haseltine,W