Rosmarinic acid in Prunella vulgaris ethanol extract inhibits lipopolysaccharide-induced prostaglandin E2 and nitric oxide in RAW 264.7 mouse macrophages.
Rosmarinic acid in Prunella vulgaris ethanol extract inhibits lipopolysaccharide-induced prostaglandin E2 and nitric oxide in RAW 264.7 mouse macrophages.
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DOI：
10.1021/jf9023728
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发表时间：
2009-11-25
影响因子：
6.1
通讯作者：
Birt DF
中科院分区：
文献类型：
--
作者：
Huang N;Hauck C;Yum MY;Rizshsky L;Widrlechner MP;McCoy JA;Murphy PA;Dixon PM;Nikolau BJ;Birt DF
夏枯草已被用于治疗炎症相关的条件了几个世纪，但缺乏系统的研究，其抗炎活性，并没有具体的活性成分已被确定。在这项研究中，将四种P. vulgaris品种的水和乙醇提取物应用于RAW 264.7小鼠巨噬细胞，并且乙醇提取物在30 μg/mL下显著抑制脂多糖（LPS）刺激的前列腺素E2（PGE 2）和一氧化氮（NO）的产生而不影响细胞活力。筛选来自不同品种的P. vulgaris的提取物的抗炎活性，以鉴定具有最大活性的品种。选定提取物对PGE 2和NO产生的抑制具有剂量依赖性，在低至10 μg/mL的浓度下即可观察到显着效果。从活性成分保藏号艾姆斯27664中对乙醇提取物进行分级，表明级分3和5可能是总体活性的主要贡献者。迷迭香酸（RA）的含量被发现独立抑制炎症反应，但它只能部分解释提取物的活性。LPS诱导的环氧合酶-2（考克斯-2）和一氧化氮合酶（iNOS）蛋白表达均被夏枯草乙醇提取物减弱，而RA仅抑制考克斯-2的表达。
Prunella vulgaris has been used therapeutically for inflammation related conditions for centuries, but systematic studies of its anti-inflammatory activity are lacking and no specific active components have been identified. In this study, water and ethanol extracts of four P. vulgaris accessions were applied to RAW264.7 mouse macrophages and the ethanol extracts significantly inhibited lipopolysaccharide (LPS)-stimulated prostaglandin E2 (PGE2) and nitric oxide (NO) production at 30 μg/mL without affecting cell viability. Extracts from different accessions of P. vulgaris were screened for anti-inflammatory activity to identify accessions with the greatest activity. The inhibition of PGE2 and NO production by selected extracts was dose-dependent, with significant effects seen at concentrations as low as 10 μg/mL. Fractionation of ethanol extracts from the active accession, Ames 27664, suggested fractions 3 and 5 as possible major contributors to the overall activity. Rosmarinic acid (RA) content in P. vulgaris was found to independently inhibit inflammatory response, but it only partially explained the extracts' activity. LPS-induced cyclooxyginase-2 (COX-2) and nitric oxide synthase (iNOS) protein expression were both attenuated by P. vulgaris ethanol extracts, while RA only inhibited COX-2 expression.
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影响因子：
4.3
作者：
Han, Eun Hee;Choi, Jae Ho;Jeong, Hye Gwang
通讯作者：
Jeong, Hye Gwang
影响因子：
6.1
作者：
Hammer, Kimberly D. P.;Hillwig, Matthew L.;Birt, Diane F.
通讯作者：
Birt, Diane F.
影响因子：
64.8
作者：
Foster, Simmie L.;Hargreaves, Diana C.;Medzhitov, Ruslan
通讯作者：
Medzhitov, Ruslan
影响因子：
7.2
作者：
Psotová, J;Kolár, M;Ulrichová, J
通讯作者：
Ulrichová, J
影响因子：
6.1
作者：
Schmitt, LA;Yi, L;Birt, DF
通讯作者：
Birt, DF