Site-directed immobilization of antibodies onto blood contacting grafts for enhanced endothelial cell adhesion and proliferation
Site-directed immobilization of antibodies onto blood contacting grafts for enhanced endothelial cell adhesion and proliferation
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将抗体定点固定到血液接触移植物上，以增强内皮细胞粘附和增殖
DOI：
10.1039/c1sm05086a
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发表时间：
2011-08
期刊：
影响因子：
3.4
通讯作者：
Yin, Min
中科院分区：
文献类型：
--
作者：
Qian, Jiangchao;Liu, Changsheng;Yuan, Yuan;Yin, Min
对内皮细胞（EC）和循环内皮祖细胞（EPC）的标志物或受体表现出高亲和力的抗体的固定被证明是加速内皮化并由此降低血液接触移植物的血栓形成的有效策略。在这里，我们开发了一种新的高碘酸盐氧化（PO）定点固定方法，以更好地控制抗体的方向，并保留其免疫活性。在该PO定点路线中，316L不锈钢（316LSS）模型基底首先用乙烯乙酸乙烯酯涂覆，然后进行氧等离子体处理和硅烷官能化。然后将高碘酸盐氧化的抗CD 34模型抗体固定化。XPS测量表明抗-CD 34的成功固定。固定化抗体保留了其生物活性，并表现出更好的捕获效率（约3倍，与传统的戊二醛表面处理相比）的特异性抗原。因此，内皮细胞的附着，4小时和12小时的培养后，分别增加了93%和116%。细胞在这些抗体包被的基质上生长得更好，具有快速的汇合周期（约12小时）。进一步的研究表明，这种PO位点定向方法能够减少血液凝固。因此，这里开发的PO位点定向途径是一种有前途的策略，用于以受控的方向和更高的生物活性来标记抗体，以用于心血管植入物的快速再内皮化。
Immobilization of antibodies, which exhibit high affinity towards the markers or receptors of endothelial cells (ECs) and circulating endothelial progenitor cells (EPCs), is proven to be an effective strategy to accelerate endothelialization and thereby lower the thrombosis of blood contacting grafts. Here, we have developed a new periodate-oxidized (PO) site-directed immobilization approach to better control the orientation of antibodies and retain their immunoactivity. In this PO site-directed route, the 316L stainless steel (316LSS) model substrate was first coated with ethylene vinylacetate and followed by oxygen plasma treatment and silane functionalization. Then the periodate-oxidized anti-CD34 model antibodies were immobilized. XPS measurements indicated the successful immobilization of the anti-CD34. The immobilized antibodies retained their bioactivity and exhibited better capturing efficiency (increase of about 3-fold compared with the conventional glutaraldehyde surface treatment) of specific antigens. Consequently, the endothelial cell attachment, after 4 h and 12 h cultivation, increased 93% and 116%, respectively. Cells grew better on these antibody-coated substrates with a quick confluent cycle (∼12 h). Further studies showed that this PO site-directed approach was able to reduce blood coagulation. Therefore, this PO site-directed route developed here is a promising strategy to immobilize antibodies with controlled orientation and higher bioactivity for rapid re-endothelialization of the cardiovascular implants.
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影响因子：
14
作者：
Meng, Sheng;Liu, Zongjun;Ge, Junbo
通讯作者：
Ge, Junbo
影响因子：
--
作者：
S. Silber
通讯作者：
S. Silber
影响因子：
8.5
作者：
S. Rashid;H. Salacinski;B. J. Fuller;G. Hamilton;A. Seifalian
通讯作者：
S. Rashid;H. Salacinski;B. J. Fuller;G. Hamilton;A. Seifalian
影响因子：
7.3
作者：
Middleton, J;Americh, L;Girard, JP
通讯作者：
Girard, JP
影响因子：
3.3
作者：
Xu, H;Lu, JR;Williams, DE
通讯作者：
Williams, DE