Comparison of the transcriptomes of American chestnut (Castanea dentata) and Chinese chestnut (Castanea mollissima) in response to the chestnut blight infection.
Comparison of the transcriptomes of American chestnut (Castanea dentata) and Chinese chestnut (Castanea mollissima) in response to the chestnut blight infection.
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DOI：
10.1186/1471-2229-9-51
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发表时间：
2009-05-09
影响因子：
5.3
通讯作者：
Carlson JE
中科院分区：
文献类型：
--
作者：
Barakat A;DiLoreto DS;Zhang Y;Smith C;Baier K;Powell WA;Wheeler N;Sederoff R;Carlson JE
美国板栗（Castanea dentata）在20世纪初受到一种外来病原体的破坏。这种栗树枯萎病是由栗疫病菌引起的，栗疫病菌是一种真菌，它会感染栗树的茎组织，并通过环剥杀死栗树。由于该物种的巨大经济和生态价值，世纪以来已经做出了重大努力来对抗这种疾病，但直到最近才开始关注基因组学方法。在壳斗科项目的基因组工具开发之前，该物种的公共数据库中可用的基因组资源仅限于几百个EST。为了鉴定与抗C.寄生物，我们已经测序的转录组从真菌感染和健康的茎组织收集枯萎病敏感的美国板栗和抗枯萎病的中国板栗（板栗）树使用超高通量焦磷酸测序。我们产生了超过100万个454读段，总计超过2.5亿bp，从中我们从C. mollissima和C. dentata分别。根据GO注释，unigenes的功能涵盖了多种分子功能和生物学过程，其中我们鉴定了大量与抗胁迫和对生物刺激的响应相关的基因。计算机表达分析表明，许多应激反应unigenes在美国和中国板栗的溃疡组织中表达比健康茎组织中表达更多。比较分析还确定了属于不同途径的植物防御生物胁迫，差异表达在美国或中国板栗溃疡组织的基因。我们的研究结果是确定了一个大的cDNA unigenes从美国板栗和中国板栗。这项研究的EST和unigenes构成了一个重要的资源，科学界有兴趣在板栗和其他物种中发现参与各种生物过程的基因。板栗溃疡病与健康茎中差异表达的许多防御相关基因的鉴定为板栗枯萎病抗性的发展和研究树木对坏死性病原体的反应途径提供了许多新的候选基因。我们还确定了几个候选基因，可能强调美国板栗和中国板栗之间的抗栗疫病的差异。
American chestnut (Castanea dentata) was devastated by an exotic pathogen in the beginning of the twentieth century. This chestnut blight is caused by Cryphonectria parasitica, a fungus that infects stem tissues and kills the trees by girdling them. Because of the great economic and ecological value of this species, significant efforts have been made over the century to combat this disease, but it wasn't until recently that a focused genomics approach was initiated. Prior to the Genomic Tool Development for the Fagaceae project, genomic resources available in public databases for this species were limited to a few hundred ESTs. To identify genes involved in resistance to C. parasitica, we have sequenced the transcriptome from fungal infected and healthy stem tissues collected from blight-sensitive American chestnut and blight-resistant Chinese chestnut (Castanea mollissima) trees using ultra high throughput pyrosequencing. We produced over a million 454 reads, totaling over 250 million bp, from which we generated 40,039 and 28,890 unigenes in total from C. mollissima and C. dentata respectively. The functions of the unigenes, from GO annotation, cover a diverse set of molecular functions and biological processes, among which we identified a large number of genes associated with resistance to stresses and response to biotic stimuli. In silico expression analyses showed that many of the stress response unigenes were expressed more in canker tissues versus healthy stem tissues in both American and Chinese chestnut. Comparative analysis also identified genes belonging to different pathways of plant defense against biotic stresses that are differentially expressed in either American or Chinese chestnut canker tissues. Our study resulted in the identification of a large set of cDNA unigenes from American chestnut and Chinese chestnut. The ESTs and unigenes from this study constitute an important resource to the scientific community interested in the discovery of genes involved in various biological processes in Chestnut and other species. The identification of many defense-related genes differentially expressed in canker vs. healthy stem in chestnuts provides many new candidate genes for developing resistance to the chestnut blight and for studying pathways involved in responses of trees to necrotrophic pathogens. We also identified several candidate genes that may underline the difference in resistance to Cryphonectria parasitica between American chestnut and Chinese chestnut.
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影响因子：
3.5
作者：
Lee, MW;Qi, M;Yang, YO
通讯作者：
Yang, YO
影响因子：
--
作者：
BREWER, LG
通讯作者：
BREWER, LG
DOI：
10.1139/cjfr-27-11-1805
发表时间：
1997-11-01
期刊：
CANADIAN JOURNAL OF FOREST RESEARCH-REVUE CANADIENNE DE RECHERCHE FORESTIERE
影响因子：
--
作者：
Carraway, DT;Merkle, SA
通讯作者：
Merkle, SA
影响因子：
11.6
作者：
Chen, WQ;Provart, NJ;Zhu, T
通讯作者：
Zhu, T
影响因子：
3.2
作者：
Kubisiak, TL;Hebard, FV;Doudrick, RL
通讯作者：
Doudrick, RL