Breakpoint structure of the Anopheles gambiae 2Rb chromosomal inversion.
Breakpoint structure of the Anopheles gambiae 2Rb chromosomal inversion.
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DOI：
10.1186/1475-2875-9-293
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发表时间：
2010-10-25
期刊：
影响因子：
3
通讯作者：
Collins FH
中科院分区：
文献类型：
--
作者：
Lobo NF;Sangaré DM;Regier AA;Reidenbach KR;Bretz DA;Sharakhova MV;Emrich SJ;Traore SF;Costantini C;Besansky NJ;Collins FH
冈比亚按蚊2号染色体倒位的替代排列是种群结构的重要来源，与环境异质性的适应有关。对它们的起源和维持负责的力量还不完全清楚。反转断点的分子表征有助于深入了解反转断点是如何产生的，并为未来研究中有用的分子核型分析方法的发展提供了基础。2RB的细胞学断裂点附近区域的序列比较允许分子描绘断裂点边界。对AN中标准的2r+b排列方式进行了比较。冈比亚按蚊的参考基因组和倒置的2RB排列。冈比亚亚种M和S的基因组组装。在设计聚合酶链式反应诊断试验时，利用了不同2RB排列之间的序列差异，并根据已知的实验室菌落和来自马里和喀麦隆的现场采集样本的染色体带型进行了评估。7.55Mb 2Rb倒位的断裂点两侧是相同的短(72bp)随机组织序列的广泛运行，这可能是染色体断裂和重排的原因。分子诊断分析表明，2Rb在AN中具有单一共同来源。冈比亚及其近亲种阿拉伯按蚊，以及标准排列(2R+b)可能通过断点重复使用出现了两次。分子诊断在实验室群体中应用是可靠的，但在自然群体中其准确性较低。2RB断裂点区域两侧的复杂重复序列可能容易出现结构和序列水平的不稳定性。2RB分子诊断在基于实验室群体的研究中立即得到应用，但它在自然种群中的有效性还有待于补充分子工具的开发。
Alternative arrangements of chromosome 2 inversions in Anopheles gambiae are important sources of population structure, and are associated with adaptation to environmental heterogeneity. The forces responsible for their origin and maintenance are incompletely understood. Molecular characterization of inversion breakpoints provides insight into how they arose, and provides the basis for development of molecular karyotyping methods useful in future studies. Sequence comparison of regions near the cytological breakpoints of 2Rb allowed the molecular delineation of breakpoint boundaries. Comparisons were made between the standard 2R+b arrangement in the An. gambiae PEST reference genome and the inverted 2Rb arrangements in the An. gambiae M and S genome assemblies. Sequence differences between alternative 2Rb arrangements were exploited in the design of a PCR diagnostic assay, which was evaluated against the known chromosomal banding pattern of laboratory colonies and field-collected samples from Mali and Cameroon. The breakpoints of the 7.55 Mb 2Rb inversion are flanked by extensive runs of the same short (72 bp) tandemly organized sequence, which was likely responsible for chromosomal breakage and rearrangement. Application of the molecular diagnostic assay suggested that 2Rb has a single common origin in An. gambiae and its sibling species, Anopheles arabiensis, and also that the standard arrangement (2R+b) may have arisen twice through breakpoint reuse. The molecular diagnostic was reliable when applied to laboratory colonies, but its accuracy was lower in natural populations. The complex repetitive sequence flanking the 2Rb breakpoint region may be prone to structural and sequence-level instability. The 2Rb molecular diagnostic has immediate application in studies based on laboratory colonies, but its usefulness in natural populations awaits development of complementary molecular tools.
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DOI：
10.1016/0035-9203(74)90035-2
发表时间：
1974-01-01
影响因子：
2.2
作者：
WHITE, GB
通讯作者：
WHITE, GB
影响因子：
3.7
作者：
Coulibaly, Mamadou B.;Lobo, Neil F.;Besansky, Nora J.
通讯作者：
Besansky, Nora J.
DOI：
10.1073/pnas.95.21.12444
发表时间：
1998-10-13
影响因子：
11.1
作者：
Mathiopoulos, KD;Della Torre, A;Coluzzi, M
通讯作者：
Coluzzi, M
影响因子：
3.3
作者：
White, Bradley J.;Cheng, Changde;Besansky, Nora J.
通讯作者：
Besansky, Nora J.
DOI：
10.1073/pnas.0509683103
发表时间：
2006-04-18
影响因子：
11.1
作者：
Sharakhov, IV;White, BJ;Besansky, NJ
通讯作者：
Besansky, NJ