Comparative distribution of human and avian type sialic acid influenza receptors in the pig.
Comparative distribution of human and avian type sialic acid influenza receptors in the pig.
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DOI：
10.1186/1746-6148-6-4
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发表时间：
2010-01-27
影响因子：
2.6
通讯作者：
Chang KC
中科院分区：
文献类型：
--
作者：
Nelli RK;Kuchipudi SV;White GA;Perez BB;Dunham SP;Chang KC
流感感染的一个主要决定因素是易感宿主细胞上存在病毒受体，病毒血凝素能够与之结合。禽病毒优先与唾液酸α 2，3-半乳糖（SAα 2，3-Gal）连接的受体结合，而人病毒株与唾液酸α 2，6-半乳糖（SAα 2，6-Gal）连接的受体结合。迄今为止，尚未发表关于SA受体在猪中分布的详细描述，猪是对禽流感和人流感亚型易感的模型宿主，因此具有病毒重配的潜力。采用凝集素-黑接骨木凝集素（SNA）和怀槐凝集素（Maackia amurensis agglutinins，MAA Ⅱ）结合法，研究了SAα 2，3-GalG（1-3）GalNAc和SAα 2，6-Gal受体在正常断奶仔猪主要器官中的相对表达和空间分布。SAα 2，3-Gal和SAα 2，6-Gal受体在检查的主要猪器官（气管、肺、肝、肾、脾、心脏、骨骼肌、大脑、小肠和结肠）中广泛检测到。此外，两种SA受体在猪呼吸道中的分布与已发表的人类呼吸道数据非常相似。除肠道外，猪和人的其他主要器官中SA受体的表达模式相似。与人类肠道中流感受体缺乏的有限报道不同，我们发现猪中SAα 2，3-Gal和SAα 2，6-Gal受体从十二指肠到结肠的存在增加。SAα 2，3-Gal和SAα 2，6-Gal受体在检查的主要器官中广泛存在，表明每个主要器官可能允许流感病毒进入或感染。猪和人之间SA表达模式的高度相似性，特别是在呼吸道中，表明猪并不比人更可能成为病毒重配的潜在宿主。我们的发现相对丰富的SA受体在猪肠道中强调了需要澄清的SA受体在人类肠道中的存在。
A major determinant of influenza infection is the presence of virus receptors on susceptible host cells to which the viral haemagglutinin is able to bind. Avian viruses preferentially bind to sialic acid α2,3-galactose (SAα2,3-Gal) linked receptors, whereas human strains bind to sialic acid α2,6-galactose (SAα2,6-Gal) linked receptors. To date, there has been no detailed account published on the distribution of SA receptors in the pig, a model host that is susceptible to avian and human influenza subtypes, thus with potential for virus reassortment. We examined the relative expression and spatial distribution of SAα2,3-GalG(1-3)GalNAc and SAα2,6-Gal receptors in the major organs from normal post-weaned pigs by binding with lectins Maackia amurensis agglutinins (MAA II) and Sambucus nigra agglutinin (SNA) respectively. Both SAα2,3-Gal and SAα2,6-Gal receptors were extensively detected in the major porcine organs examined (trachea, lung, liver, kidney, spleen, heart, skeletal muscle, cerebrum, small intestine and colon). Furthermore, distribution of both SA receptors in the pig respiratory tract closely resembled the published data of the human tract. Similar expression patterns of SA receptors between pig and human in other major organs were found, with exception of the intestinal tract. Unlike the limited reports on the scarcity of influenza receptors in human intestines, we found increasing presence of SAα2,3-Gal and SAα2,6-Gal receptors from duodenum to colon in the pig. The extensive presence of SAα2,3-Gal and SAα2,6-Gal receptors in the major organs examined suggests that each major organ may be permissive to influenza virus entry or infection. The high similarity of SA expression patterns between pig and human, in particular in the respiratory tract, suggests that pigs are not more likely to be potential hosts for virus reassortment than humans. Our finding of relative abundance of SA receptors in the pig intestines highlights a need for clarification on the presence of SA receptors in the human intestinal tract.
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作者：
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通讯作者：
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