Genetic diversity among Plasmodium falciparum field isolates in Pakistan measured with PCR genotyping of the merozoite surface protein 1 and 2.
Genetic diversity among Plasmodium falciparum field isolates in Pakistan measured with PCR genotyping of the merozoite surface protein 1 and 2.
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DOI：
10.1186/1475-2875-9-1
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发表时间：
2010-01-01
期刊：
影响因子：
3
通讯作者：
Beg MA
中科院分区：
文献类型：
--
作者：
Ghanchi NK;Mårtensson A;Ursing J;Jafri S;Bereczky S;Hussain R;Beg MA
恶性疟原虫的遗传多样性在世界各地得到了广泛的研究。然而，从巴基斯坦获得的数据有限。本研究旨在利用裂殖子表面蛋白1(MSP-1)和裂殖子表面蛋白2(MSP-2)这两个高度多态的遗传标记，建立巴基斯坦恶性疟原虫野外分离株的分子特征。2005年10月至2007年10月，在卡拉奇Aga Khan大学医院或其位于巴基斯坦信德省和俾路支省的收集单位收集了244份有症状的血液玻片确诊恶性疟原虫感染患者的血液样本。针对恶性疟原虫MSP-1和MSP-2基因片段2和3片段的套式聚合酶链式反应(PCR)扩增产物进行长度多态凝胶电泳法，分析恶性疟原虫各自的等位基因家族Ki、MAD 20、RO33和FC27、3D7/IC的遗传多样性。总共有238/244名患者(98%)至少有一个遗传标记的聚合酶链式反应结果为阳性；其余6名患者被排除在分析之外。大多数患者有单克隆性感染。只有56/231(24%)和51/236(22%)携带恶性疟原虫MSP-1和MSP-2多个基因型。基因分型总数估计为25个MSP-1(12个KI；8个MAD20；5个RO33)和33个MSP-2(14个FC27；19个3D7/IC)。这是巴基斯坦首次报道恶性疟原虫野外分离株与感染多样性有关的分子特征。这项研究中发现的遗传多样性和等位基因分布与印度和疟疾流行率较低的东南亚国家之前的报告相似。
The genetic diversity of Plasmodium falciparum has been extensively studied in various parts of the world. However, limited data are available from Pakistan. This study aimed to establish molecular characterization of P. falciparum field isolates in Pakistan measured with two highly polymorphic genetic markers, i.e. the merozoite surface protein 1 (msp-1)and 2 (msp-2). Between October 2005 and October 2007, 244 blood samples from patients with symptomatic blood-slide confirmed P. falciparum mono-infections attending the Aga Khan University Hospital, Karachi, or its collection units located in Sindh and Baluchistan provinces, Pakistan were collected. The genetic diversity of P. falciparum was analysed by length polymorphism following gel electrophoresis of DNA products from nested polymerase chain reactions (PCR) targeting block 2 of msp-1 and block 3 of msp-2, including their respective allelic families KI, MAD 20, RO33, and FC27, 3D7/IC. A total of 238/244 (98%) patients had a positive PCR outcome in at least one genetic marker; the remaining six were excluded from analysis. A majority of patients had monoclonal infections. Only 56/231 (24%) and 51/236 (22%) carried multiple P. falciparum genotypes in msp-1 and msp-2, respectively. The estimated total number of genotypes was 25 msp-1 (12 KI; 8 MAD20; 5 RO33) and 33 msp-2 (14 FC27; 19 3D7/IC). This is the first report on molecular characterization of P. falciparum field isolates in Pakistan with regards to multiplicity of infection. The genetic diversity and allelic distribution found in this study is similar to previous reports from India and Southeast Asian countries with low malaria endemicity.
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影响因子：
3.3
作者：
Peyerl-Hoffmann, G;Jelinek, T;von Sonnenburg, F
通讯作者：
von Sonnenburg, F
DOI：
10.1073/pnas.88.5.1751
发表时间：
1991-03-01
影响因子：
11.1
作者：
SMYTHE, JA;COPPEL, RL;ANDERS, RF
通讯作者：
ANDERS, RF
影响因子：
3
作者：
Joshi, Hema;Valecha, Neena;Dash, Aditya P.
通讯作者：
Dash, Aditya P.
影响因子：
6.4
作者：
Beck, HP;Felger, I;Tanner, M
通讯作者：
Tanner, M
影响因子：
3.4
作者：
Warhurst, DC;Williams, JE
通讯作者：
Williams, JE