A novel immunodiagnostic assay to detect serum antibody response against selected soluble egg antigen fractions from Schistosoma japonicum.
A novel immunodiagnostic assay to detect serum antibody response against selected soluble egg antigen fractions from Schistosoma japonicum.
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一种检测日本血吸虫可溶性卵抗原组分血清抗体反应的新型免疫诊断方法
DOI：
10.1371/journal.pone.0044032
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发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Lu S
中科院分区：
文献类型：
--
作者：
Zhu Y;Hua W;Xu M;He W;Wang X;Dai Y;Zhao S;Tang J;Wang S;Lu S
日本血吸虫病对中国的公共卫生仍然是一个真实的威胁。目前使用的免疫诊断方法灵敏度高，具有一定的特异性，但它们都使用复杂的粗抗原，基于对寄生虫体特异性抗体的检测，并已显示与其他寄生虫病交叉反应。因此，这些试验不能用于评价化疗疗效。发展高灵敏度和高特异性的免疫诊断技术，可以监测特异性抗体的下降。日本血吸虫病作为流行地区控制血吸虫病的持续战略的一部分将是极其宝贵的。在这里，我们报告的可溶性虫卵抗原（SEA）的抗体消失7周后有效治疗的独特部分抗原的鉴定。此外，我们使用这些SEA组分开发具有高灵敏度和特异性的改良测定法。方法/主要发现SEA的S。通过在非还原条件下使用7. 5% SDS-PAGE的电泳将日本血吸虫分级分离。收集处理后不久抗体下降的SEA组分抗原，并用作检测抗原以建立FA-ELISA。用急、慢性血吸虫病患者、健康人及其他寄生虫病患者血清评价其敏感性和特异性。并在治疗前、后不同时间点检测慢性血吸虫病患者的血清，以评价其化疗效果。结论：本研究建立的FA-ELISA方法具有较高的敏感性和特异性，交叉反应性低，可作为评价化疗对沙门氏菌疗效的有效工具。山茱萸
Background Schistosomiasis japonica remains a real threat to public health in China. The currently used immunodiagnostic assays are sensitive and have a certain degree of specificity, however, they all use complex crude antigens, are based on detection of schistosome-specific antibodies, and have been shown to cross-react with other parasitic diseases. Therefore, these assays cannot be used to evaluate chemotherapy efficacy. The development of highly sensitive and highly specific immunodiagnostic techniques that can monitor the decline of antibodies specific for S. japonica will be extremely valuable as part of the ongoing strategy to control schistosomiasis in endemic areas. Here we report on the identification of unique fraction antigens of soluble egg antigen (SEA) to which the antibodies disappear 7 weeks after effective treatment. Furthermore, we use these SEA fractions to develop a modified assay with both high sensitivity and specificity. Methodology/Principal Findings SEA of S. japonicum was fractionated by electrophoresis using 7.5% SDS-PAGE under non-reducing conditions. The SEA fraction antigens to which antibodies were decreased soon after treatment were collected and used as the detection antigens to establish the FA-ELISA. Sera from patients with acute and chronic schistosomiasis infection, healthy people, and those with other parasitic diseases, were used to evaluate their sensitivity and specificity. Furthermore, sera from patients with chronic schistosomiasis infection were evaluated before and after treatment at different time points to evaluate their chemotherapeutic efficacy. Conclusion/Significance We demonstrated that this novel FA-ELISA provided high sensitivity and specificity, with very low cross-reactivity, and can serve as an effective tool to determine the efficacy of chemotherapy against S. japonicum.
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影响因子：
2.7
作者：
Zhu, YC;Socheat, D;Bergquist, R
通讯作者：
Bergquist, R
影响因子：
2.8
作者：
BERGQUIST, NR
通讯作者：
BERGQUIST, NR
影响因子：
2.7
作者：
Wu, GL
通讯作者：
Wu, GL
影响因子：
2.2
作者：
Zhu, YC;He, W;Chao, GQ
通讯作者：
Chao, GQ