Pueraria mirifica Exerts Estrogenic Effects in the Mammary Gland and Uterus and Promotes Mammary Carcinogenesis in Donryu Rats.
Pueraria mirifica Exerts Estrogenic Effects in the Mammary Gland and Uterus and Promotes Mammary Carcinogenesis in Donryu Rats.
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DOI：
10.3390/toxins8110275
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发表时间：
2016-11-04
期刊：
影响因子：
4.2
通讯作者：
Wanibuchi H
中科院分区：
文献类型：
--
作者：
Kakehashi A;Yoshida M;Tago Y;Ishii N;Okuno T;Gi M;Wanibuchi H
葛根（PM）是一种植物，其干燥和粉末状的块根现在被广泛用于恢复活力的制剂中，以促进男性和女性的青春，可能具有主要的雌激素影响。在本研究中，我们研究了不同剂量的PM对雌性东流大鼠乳腺和子宫内膜癌变的调节作用。首先，在低植物雌激素饮食中给药0.03%，0.3%和3%剂量的PM 2周后，卵巢切除的动物子宫重量显著增加。其次，在7,12-二甲基苯[a]蒽（DMBA）启动后，以3%剂量的PM应用于未手术大鼠4周，导致乳腺细胞增殖显著升高。在第三个实验中，在乳房和子宫内膜癌开始发生后，5周龄的未手术动物在青春期后分别给予0.3% （200 mg/kg体重(b.w.)/天）PM，持续36周，DMBA和n-乙基-n ' -硝基-n -亚硝基胍(N-ethyl-N ' -硝基-n -亚硝基胍（ENNG），导致乳腺腺癌发病率显著增加。子宫内膜不典型增生的多重性也显著增加。此外，0.3%剂量的PM，更明显的是，1%剂量的PM诱导子宫壁扩张、出血和炎症。综上所述，东柳大鼠青春期后长期服用PM对乳腺和子宫具有雌激素作用，200 mg/kg体重/天剂量可促进DMBA引发的乳腺癌发生。
Pueraria mirifica (PM), a plant whose dried and powdered tuberous roots are now widely used in rejuvenating preparations to promote youthfulness in both men and women, may have major estrogenic influence. In this study, we investigated modifying effects of PM at various doses on mammary and endometrial carcinogenesis in female Donryu rats. Firstly, PM administered to ovariectomized animals at doses of 0.03%, 0.3%, and 3% in a phytoestrogen-low diet for 2 weeks caused significant increase in uterus weight. Secondly, a 4 week PM application to non-operated rats at a dose of 3% after 7,12-dimethylbenz[a]anthracene (DMBA) initiation resulted in significant elevation of cell proliferation in the mammary glands. In a third experiment, postpubertal administration of 0.3% (200 mg/kg body weight (b.w.)/day) PM to 5-week-old non-operated animals for 36 weeks following initiation of mammary and endometrial carcinogenesis with DMBA and N-ethyl-N′-nitro-N-nitrosoguanidine (ENNG), respectively, resulted in significant increase of mammary adenocarcinoma incidence. A significant increase of endometrial atypical hyperplasia multiplicity was also observed. Furthermore, PM at doses of 0.3%, and more pronouncedly, at 1% induced dilatation, hemorrhage and inflammation of the uterine wall. In conclusion, postpubertal long-term PM administration to Donryu rats exerts estrogenic effects in the mammary gland and uterus, and at a dose of 200 mg/kg b.w./day was found to promote mammary carcinogenesis initiated by DMBA.
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影响因子：
3.7
作者：
Kakehashi A;Ishii N;Fujioka M;Doi K;Gi M;Wanibuchi H
通讯作者：
Wanibuchi H
影响因子：
2.2
作者：
Shimokawa, Satoko;Kumamoto, Takuya;Chansakaow, Sunee
通讯作者：
Chansakaow, Sunee
影响因子：
4.8
作者：
Lucki, Natasha C.;Sewer, Marion B.
通讯作者：
Sewer, Marion B.
影响因子：
8.1
作者：
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通讯作者：
Malaivijitnond, Suchinda
影响因子：
4.9
作者：
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通讯作者：
Picha, Porntipa