The beta4 integrin interactor p27(BBP/eIF6) is an essential nuclear matrix protein involved in 60S ribosomal subunit assembly.
The beta4 integrin interactor p27(BBP/eIF6) is an essential nuclear matrix protein involved in 60S ribosomal subunit assembly.
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DOI：
10.1083/jcb.144.5.823
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发表时间：
1999-03-08
期刊：
影响因子：
--
通讯作者：
Biffo S
中科院分区：
文献类型：
--
作者：
Sanvito F;Piatti S;Villa A;Bossi M;Lucchini G;Marchisio PC;Biffo S
p27 BBP/eIF 6是一种进化上保守的蛋白质，最初被鉴定为p27 BBP，整合素β4的胞质结构域的相互作用物，并且独立地作为推定的翻译起始因子eIF 6。为了建立p27 BBP/eIF 6的体内功能，研究了其在哺乳动物细胞中的拓扑分布，并研究了在芽殖酵母酿酒酵母中破坏相应基因的影响。在含有β4整合素的上皮细胞中，p27 BBP/eIF 6存在于细胞质中，并以类似于β4整合素的模式富集于半桥粒。令人惊讶的是，在β4整联蛋白亚基存在和不存在的情况下，p27 BBP/eIF 6位于核仁中并与核基质相关。删除IIH S。酿酒酵母p27 BBP/eIF 6同源基因是致死性的，相应基因产物的缺失与游离核糖体60 S亚基水平的急剧下降有关。此外，人p27 BBP/eIF 6可以挽救iihΔ酵母突变的致死效应。体内获得的数据表明，p27 BBP/eIF 6在核糖体生物合成或组装中而不是在翻译中具有进化上保守的功能。与β4整联蛋白亚基相关的进一步功能可能在高等真核细胞中特异性进化。
p27BBP/eIF6 is an evolutionarily conserved protein that was originally identified as p27BBP, an interactor of the cytoplasmic domain of integrin β4 and, independently, as the putative translation initiation factor eIF6. To establish the in vivo function of p27BBP/eIF6, its topographical distribution was investigated in mammalian cells and the effects of disrupting the corresponding gene was studied in the budding yeast, Saccharomyces cerevisiae. In epithelial cells containing β4 integrin, p27BBP/eIF6 is present in the cytoplasm and enriched at hemidesmosomes with a pattern similar to that of β4 integrin. Surprisingly, in the absence and in the presence of the β4 integrin subunit, p27BBP/eIF6 is in the nucleolus and associated with the nuclear matrix. Deletion of the IIH S. cerevisiae gene, encoding the yeast p27BBP/eIF6 homologue, is lethal, and depletion of the corresponding gene product is associated with a dramatic decrease of the level of free ribosomal 60S subunit. Furthermore, human p27BBP/eIF6 can rescue the lethal effect of the iihΔ yeast mutation. The data obtained in vivo suggest an evolutionarily conserved function of p27BBP/eIF6 in ribosome biogenesis or assembly rather than in translation. A further function related to the β4 integrin subunit may have evolved specifically in higher eukaryotic cells.
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影响因子：
7.5
作者：
Chicurel, ME;Chen, CS;Ingber, DE
通讯作者：
Ingber, DE
影响因子：
5.3
作者：
Kressler, D;De la Cruz, J;Linder, P
通讯作者：
Linder, P
影响因子：
64.8
作者：
Chicurel, ME;Singer, RH;Ingber, DE
通讯作者：
Ingber, DE
影响因子：
7.5
作者：
Howe, A;Aplin, AE;Juliano, RL
通讯作者：
Juliano, RL
DOI：
10.1006/bbrc.1998.9609
发表时间：
1998-11-09
影响因子：
3.1
作者：
Cho, SH;Cho, JJ;Oh, CK
通讯作者：
Oh, CK