A molecular neuroanatomical study of the developing human neocortex from 8 to 17 postconceptional weeks revealing the early differentiation of the subplate and subventricular zone.
A molecular neuroanatomical study of the developing human neocortex from 8 to 17 postconceptional weeks revealing the early differentiation of the subplate and subventricular zone.
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DOI：
10.1093/cercor/bhm184
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发表时间：
2008-07
期刊：
影响因子：
--
通讯作者：
Clowry GJ
中科院分区：
文献类型：
--
作者：
Bayatti N;Moss JA;Sun L;Ambrose P;Ward JF;Lindsay S;Clowry GJ
我们采用多种标记物的免疫组织化学方法来研究人类大脑壁不同区室的结构和可能的功能，从怀孕后 8 周 (PCW) 皮质板的形成到 17 PCW 丘脑皮质传入神经的到达。新的观察结果包括亚板以 10 PCW 的形式出现为离散的分化层，其特征是突触素和囊泡 γ-氨基丁酸转运蛋白表达也出现在边缘区，这表明这些区室甚至在丘脑皮质传入神经到达之前也可能维持自发活跃的突触网络。亚板从13 PCW扩展到17 PCW，成为最大的隔室并进一步分化，NPY神经元位于外亚板，KCC2神经元位于内亚板。谷氨酸脱羧酶和钙结合蛋白阳性抑制性神经元从 11.5 PCW 开始切向和径向迁移，大量出现向喙极。以 Ki67 表达为标志的增殖区形成了 12.5 PCW 的复杂结构，这反映在转录因子表达模式中，包括局限于内室下区和外室区的 TBR2 以及在室下区 (SVZ) 弱表达的 TBR1。 PAX6 在增殖区广泛表达，因此人类外部 SVZ 包含大量 PAX6 阳性潜在祖细胞。
We have employed immunohistochemistry for multiple markers to investigate the structure and possible function of the different compartments of human cerebral wall from the formation of cortical plate at 8 postconceptional weeks (PCW) to the arrival of thalamocortical afferents at 17 PCW. New observations include the subplate emerging as a discrete differentiated layer by 10 PCW, characterized by synaptophysin and vesicular gamma-aminobutyric acid transporter expression also seen in the marginal zone, suggesting that these compartments may maintain a spontaneously active synaptic network even before the arrival of thalamocortical afferents. The subplate expanded from 13 to 17 PCW, becoming the largest compartment and differentiated further, with NPY neurons located in the outer subplate and KCC2 neurons in the inner subplate. Glutamate decarboxylase and calretinin-positive inhibitory neurons migrated tangentially and radially from 11.5 PCW, appearing in larger numbers toward the rostral pole. The proliferative zones, marked by Ki67 expression, developed a complicated structure by 12.5 PCW reflected in transcription factor expression patterns, including TBR2 confined to the inner subventricular and outer ventricular zones and TBR1 weakly expressed in the subventricular zone (SVZ). PAX6 was extensively expressed in the proliferative zones such that the human outer SVZ contained a large reservoir of PAX6-positive potential progenitor cells.
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影响因子：
25
作者：
Bystron, Irina;Rakic, Pasko;Blakemore, Colin
通讯作者：
Blakemore, Colin
DOI：
10.1073/pnas.81.20.6526
发表时间：
1984-01-01
期刊：
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES
影响因子：
--
作者：
HENDRY, SHC;JONES, EG;EMSON, PC
通讯作者：
EMSON, PC
影响因子：
16.2
作者：
Hevner, RF;Shi, LM;Rubenstein, JLR
通讯作者：
Rubenstein, JLR
影响因子：
2.5
作者：
BERNHARDT, R;MATUS, A
通讯作者：
MATUS, A
影响因子：
5.3
作者：
Chan, Charles;Moore, Brian E.;Stopa, Edward G.
通讯作者：
Stopa, Edward G.