Extracellular DNA-dependent biofilm formation by Staphylococcus epidermidis RP62A in response to subminimal inhibitory concentrations of antibiotics.
Extracellular DNA-dependent biofilm formation by Staphylococcus epidermidis RP62A in response to subminimal inhibitory concentrations of antibiotics.
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DOI：
10.1016/j.resmic.2011.03.008
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发表时间：
2011-06
影响因子：
2.6
通讯作者：
Sadovskaya, Irina
中科院分区：
文献类型：
--
作者：
Kaplan, Jeffrey B.;Jabbouri, Said;Sadovskaya, Irina
关键词：
我们测量了表皮葡萄球菌在万古霉素、替加环素、利奈唑胺和新生霉素的亚最小抑菌浓度（sub-MIC）存在下形成生物膜的能力。测试了产生不同量的生物膜的六种菌株。产生最高量生物膜的三种菌株在亚MIC抗生素存在下表现出稳态或减少的生物膜形成，而产生较低量生物膜的三种菌株在亚MIC抗生素存在下表现出高达10倍增加的生物膜形成。在两种诱导型菌株（9142和456 a）中，抗生素诱导的生物膜形成被分散素B（一种降解聚-N-乙酰葡糖胺（PNAG）生物膜多糖的酶）抑制。在第三种诱导型菌株（RP 62 A）中，分散素B抑制了对亚MIC万古霉素的生物膜形成，但对亚MIC替加环素没有抑制作用。相比之下，DNA酶I有效地抑制响应于亚MIC替加环素和万古霉素的菌株RP 62 A的生物膜形成。DNase I对菌株9142和456 a中抗生素诱导的生物膜形成没有影响。我们的研究结果表明，抗生素诱导的生物膜形成在S。epidermidis是菌株依赖性和寄生虫依赖性的，S. epidermidis RP 62 A利用细胞外DNA依赖性机制来响应于亚MIC抗生素形成生物膜。
We measured the ability of Staphylococcus epidermidis to form biofilms in the presence of subminimal inhibitory (sub-MIC) concentrations of vancomycin, tigecycline, linezolid and novobiocin. Six strains that produce different amounts of biofilm were tested. The three strains that produced the highest amounts of biofilm exhibited steady-state or decreased biofilm formation in the presence of sub-MIC antibiotics, whereas the three strains that produced lower amounts of biofilm exhibited up to 10-fold-increased biofilm formation in the presence of sub-MIC antibiotics. In two of the inducible strains (9142 and 456a), antibiotic-induced biofilm formation was inhibited by dispersin B, an enzyme that degrades poly-N-acetylglucosamine (PNAG) biofilm polysaccharide. In the third inducible strain (RP62A), dispersin B inhibited biofilm formation in response to sub-MIC vancomycin, but not to sub-MIC tigecycline. In contrast, DNase I efficiently inhibited biofilm formation by strain RP62A in response to sub-MIC tigecycline and vancomycin. DNase I had no effect on antibiotic-induced biofilm formation in strains 9142 and 456a. Our findings indicate that antibiotic-induced biofilm formation in S. epidermidis is both strain- and antibiotic-dependent and that S. epidermidis RP62A utilizes an extracellular DNA-dependent mechanism to form biofilms in response to sub-MIC antibiotics.
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