First report of Cryptosporidium spp. in white yaks in China.
First report of Cryptosporidium spp. in white yaks in China.
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DOI：
10.1186/1756-3305-7-230
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发表时间：
2014-05-19
影响因子：
3.2
通讯作者：
Zhu XQ
中科院分区：
文献类型：
--
作者：
Qin SY;Zhang XX;Zhao GH;Zhou DH;Yin MY;Zhao Q;Zhu XQ
隐孢子虫是一种肠道顶复体寄生虫，可感染牦牛，导致产奶量下降和体重增加不良。白色牦牛是甘肃省天祝藏族自治县特有的牦牛品种。本研究的目的是从分子水平上确定白色牦牛隐孢子虫的感染和种类。采集甘肃省天祝藏族自治县白色牦牛粪便样品76份。用巢式PCR扩增每个样品的小亚基核糖体RNA（SSU rRNA）基因并测序。通过BLAST（http：//www.ncbi.nlm.nih.gov/BLAST/）将获得的序列与GenBank中可获得的相应隐孢子虫序列进行比较，并使用PAUP* 进行最大似然（ML）系统发育分析，确定隐孢子虫属物种。白色牦牛隐孢子虫感染率为5.26%（4/76）。种鉴定结果表明，C. andersoni和C.在三个样本中发现牛病毒（一个在11月采集，两个在9月采集）。本研究首次发现我国白色牦牛存在隐孢子虫感染，并从牦牛体内分离出两种隐孢子虫，即隐孢子虫C. andersoni和C.牛，被发现。这些发现扩展了隐孢子虫属的宿主范围，为进一步开展该特有白色牦牛隐孢子虫病的分子流行病学研究和防治提供了基础资料。
Cryptosporidium is an enteric apicomplexan parasite, which can infect yaks, leading to reduction of milk production and poor weight gain. White yak (Bos grunniens) is a unique yak breed inhabiting only in Tianzhu Tibetan Autonomous County, Gansu province, northwestern China. The objective of the present study was to molecularly determine Cryptosporidium infection and species in white yaks. Seventy-six fecal samples from white yaks in Tianzhu Tibetan Autonomous County, Gansu province were collected. The small subunit ribosomal RNA (SSU rRNA) gene of each sample was amplified using nested PCR and sequenced. The Cryptosporidium species was determined by comparison of the obtained sequences with that of corresponding Cryptosporidium sequences available in GenBank by BLAST (http://www.ncbi.nlm.nih.gov/BLAST/) and phylogenetic analysis with maximum likelihood (ML) using PAUP*. The overall prevalence of Cryptosporidium infection in white yak was 5.26% (4/76). Species identification showed C. andersoni in one sample (collected in September), and C. bovis in three samples (one collected in November and two collected in September). The present investigation revealed the existence of Cryptosporidium infection in white yaks in China, for the first time, and two Cryptosporidium species, namely C. andersoni and C. bovis, were identified. These findings extend the host range for Cryptosporidium spp., and also provide base-line information for further studies of molecular epidemiology and control of Cryptosporidium infection in the unique white yaks.
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影响因子：
3.7
作者：
Wegayehu, Teklu;Adamu, Haileeyesus;Petros, Beyene
通讯作者：
Petros, Beyene
中国西北陕西省牛的安氏隐孢子虫基因分型
影响因子：
3.7
作者：
Zhao GH;Ren WX;Gao M;Bian QQ;Hu B;Cong MM;Lin Q;Wang RJ;Qi M;Qi MZ;Zhu XQ;Zhang LX
通讯作者：
Zhang LX
影响因子：
2.6
作者：
Amer, Said;Zidan, Shereif;Xiao, Lihua
通讯作者：
Xiao, Lihua
影响因子：
2.6
作者：
Feng, Yaoyu;Ortega, Ynes;Xiao, Lihua
通讯作者：
Xiao, Lihua
影响因子：
--
作者：
Xiao, Lihua
通讯作者：
Xiao, Lihua