The Acquisition and Retention of Lumpy Skin Disease Virus by Blood-Feeding Insects Is Influenced by the Source of Virus, the Insect Body Part, and the Time since Feeding.
The Acquisition and Retention of Lumpy Skin Disease Virus by Blood-Feeding Insects Is Influenced by the Source of Virus, the Insect Body Part, and the Time since Feeding.
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DOI：
10.1128/jvi.00751-22
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发表时间：
2022-08-10
影响因子：
5.4
通讯作者：
中科院分区：
文献类型：
--
作者：
块状皮肤病病毒(LSDV)是一种引起牛严重全身疾病的痘病毒，通过节肢动物机械传播传播。这项研究量化了四种双翅目昆虫(Stomoxys calcirans、埃及伊蚊、致倦库蚊和库蚊)从皮损、正常皮肤和临床感染动物的血液中获取和保留LSDV的情况。Ae收购和保留LSDV。还对人工膜饲养系统中的埃及伊蚊进行了检测。建立了数据的数学模型，以识别影响昆虫获取和保留LSDV的参数。对于所有四种昆虫来说，与以临床上受影响动物的正常皮肤或血液为食相比，以病变为食时感染LSDV的可能性要大得多。在摄食皮肤损伤后，所有四种动物在鼻子上停留的时间相似(约9 天)，而在身体其他部位的停留时间较短，从2.2 到6.4 天不等。Ae收购和保留LSDV。埃及伊蚊在摄食含有高滴度LSDV的人造膜饲养系统后，可以与在临床感染动物的皮肤病变上饲养类似，支持使用该实验室模型作为某些动物研究的替代。这项工作表明，LSD的皮肤损伤提供了昆虫感染病毒所需的高滴度来源，从而使机械媒介传播成为可能。重要意义块状皮肤病病毒(LSDV)是牛的一种严重后果的病原体，其地理范围正在迅速扩展到欧洲和亚洲等新地区。病毒通过血源性节肢动物的机械传播促进了这种扩张。这项研究量化了四种食血昆虫对LSDV的获取和滞留，揭示了LSD的皮肤病变为昆虫获取病毒提供了必要的高滴度病毒源。含有高滴度LSDV的人工膜喂养系统被证明在用作病毒源时，与临床感染动物的皮肤损伤相当。这促进了这些以实验室为基础的系统作为一些动物研究的替代品的使用。总体而言，这项工作促进了我们对LSDV机械媒介传播的理解，并为设计更有效的疾病控制方案提供了证据。
Lumpy skin disease virus (LSDV) is a poxvirus that causes severe systemic disease in cattle and is spread by mechanical arthropod-borne transmission. This study quantified the acquisition and retention of LSDV by four species of Diptera (Stomoxys calcitrans, Aedes aegypti, Culex quinquefasciatus, and Culicoides nubeculosus) from cutaneous lesions, normal skin, and blood from a clinically affected animal. The acquisition and retention of LSDV by Ae. aegypti from an artificial membrane feeding system was also examined. Mathematical models of the data were generated to identify the parameters which influence insect acquisition and retention of LSDV. For all four insect species, the probability of acquiring LSDV was substantially greater when feeding on a lesion compared with feeding on normal skin or blood from a clinically affected animal. After feeding on a skin lesion LSDV was retained on the proboscis for a similar length of time (around 9 days) for all four species and for a shorter time in the rest of the body, ranging from 2.2 to 6.4 days. Acquisition and retention of LSDV by Ae. aegypti after feeding on an artificial membrane feeding system that contained a high titer of LSDV was comparable to feeding on a skin lesion on a clinically affected animal, supporting the use of this laboratory model as a replacement for some animal studies. This work reveals that the cutaneous lesions of LSD provide the high-titer source required for acquisition of the virus by insects, thereby enabling the mechanical vector-borne transmission. IMPORTANCE Lumpy skin disease virus (LSDV) is a high consequence pathogen of cattle that is rapidly expanding its geographical boundaries into new regions such as Europe and Asia. This expansion is promoted by the mechanical transmission of the virus via hematogenous arthropods. This study quantifies the acquisition and retention of LSDV by four species of blood-feeding insects and reveals that the cutaneous lesions of LSD provide the high titer virus source necessary for virus acquisition by the insects. An artificial membrane feeding system containing a high titer of LSDV was shown to be comparable to a skin lesion on a clinically affected animal when used as a virus source. This promotes the use of these laboratory-based systems as replacements for some animal studies. Overall, this work advances our understanding of the mechanical vector-borne transmission of LSDV and provides evidence to support the design of more effective disease control programmes.
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影响因子：
5.4
作者：
Sanz-Bernardo B;Haga IR;Wijesiriwardana N;Basu S;Larner W;Diaz AV;Langlands Z;Denison E;Stoner J;White M;Sanders C;Hawes PC;Wilson AJ;Atkinson J;Batten C;Alphey L;Darpel KE;Gubbins S;Beard PM
通讯作者：
Beard PM
影响因子：
4.2
作者：
Tuppurainen, E. S. M.;Lubinga, J. C.;Oura, C. A. L.
通讯作者：
Oura, C. A. L.
影响因子：
4.2
作者：
Gari, G.;Waret-Szkuta, A.;Roger, F.
通讯作者：
Roger, F.
影响因子：
4.3
作者：
Sevik, M.;Dogan, M.
通讯作者：
Dogan, M.
影响因子：
4.6
作者：
Aleksandr, Kononov;Olga, Byadovskaya;Alexander, Sprygin
通讯作者：
Alexander, Sprygin