Double-Network Hydrogel with Tunable Mechanical Performance and Biocompatibility for the Fabrication of Stem Cells-Encapsulated Fibers and 3D Assemble.
Double-Network Hydrogel with Tunable Mechanical Performance and Biocompatibility for the Fabrication of Stem Cells-Encapsulated Fibers and 3D Assemble.
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DOI：
10.1038/srep33462
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发表时间：
2016-09-15
影响因子：
4.6
通讯作者：
Liang Q
中科院分区：
文献类型：
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作者：
Liang Z;Liu C;Li L;Xu P;Luo G;Ding M;Liang Q
细胞包裹纤维的制备可以为组织工程和再生医学做出巨大贡献。然而，现有的方法不仅不可避免地受到细胞破坏条件和/或复杂设备的影响，而且无法获得适当的材料来满足机械和生物期望。在这项工作中，提出了一种简单的方法来制备具有机械强度和拉伸行为以及直径和微观结构可调的细胞包覆纤维。水凝胶纤维是由海藻酸盐和聚(N-异丙基丙烯酰胺)-聚乙二醇的优化组合而成，具有双网络水凝胶的特性，具有足够的刚性和柔韧性，可以无裂纹地形成平行螺旋和不同结点等多种三维结构。此外，这种水凝胶纤维具有更好的相容性，培养4天后包裹的胚胎干细胞的活性、增殖和多能标志的表达表明。这种双网络水凝胶对藻酸盐裂解酶、EDTA或更低的环境温度具有特定的快速反应，有助于纤维或纤维组件的选择性降解，以释放被包裹的细胞，用于后续的检测或治疗。
Fabrication of cell-encapsulated fibers could greatly contribute to tissue engineering and regenerative medicine. However, existing methods suffered from not only unavoidability of cell damaging conditions and/or sophisticated equipment, but also unavailability of proper materials to satisfy both mechanical and biological expectations. In this work, a simple method is proposed to prepare cell-encapsulated fibers with tunable mechanical strength and stretching behavior as well as diameter and microstructure. The hydrogel fibers are made from optimal combination of alginate and poly(N-iso-propylacrylamide)-poly(ethylene glycol), characteristics of double-network hydrogel, with enough stiffness and flexibility to create a variety of three dimensional structures like parallel helical and different knots without crack. Furthermore, such hydrogel fibers exhibit better compatibility as indicated by the viability, proliferation and expression of pluripotency markers of embryonic stem cells encapsulated after 4-day culture. The double-network hydrogel possesses specific quick responses to either of alginate lyase, EDTA or lower environmental temperature which facilitate the optional degradation of fibers or fibrous assemblies to release the cells encapsulated for subsequent assay or treatment.
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影响因子：
14
作者：
Bryant, Stephanie J.;Cuy, Janet L.;Ratner, Buddy D.
通讯作者：
Ratner, Buddy D.
影响因子：
2.9
作者：
Arnold, M;Cavalcanti-Adam, EA;Spatz, JP
通讯作者：
Spatz, JP
影响因子：
14
作者：
Ashton, Randolph S.;Banerjee, Akhilesh;Kane, Ravi S.
通讯作者：
Kane, Ravi S.
影响因子：
4.6
作者：
Grulova I;Slovinska L;Blaško J;Devaux S;Wisztorski M;Salzet M;Fournier I;Kryukov O;Cohen S;Cizkova D
通讯作者：
Cizkova D
影响因子：
38.3
作者：
通讯作者：
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