Changes in membrane-microfilament interaction in intercellular adherens junctions upon removal of extracellular Ca2+ ions.
Changes in membrane-microfilament interaction in intercellular adherens junctions upon removal of extracellular Ca2+ ions.
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DOI：
10.1083/jcb.102.5.1832
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发表时间：
1986-05
影响因子：
7.8
通讯作者：
FRANKE, WW
中科院分区：
文献类型：
--
作者：
VOLBERG, T;GEIGER, B;KARTENBECK, J;FRANKE, WW
EGTA诱导的Madin-达比牛肾上皮细胞培养基中Ca 2+离子的耗竭导致粘附蛋白型连接的快速分裂以及含黏着斑蛋白和肌动蛋白的丝束从粘附小带质膜的细胞质面脱离。这一过程通过相差显微镜，结合电子显微镜和两种蛋白质的免疫荧光定位来监测。结果表明，细胞外游离Ca 2+浓度降低到微摩尔范围后不久，肌动蛋白，连接相关带的微丝，连同长春纽蛋白丰富的交界处斑块材料，是不可逆地脱离作为一个结构单位从质膜，合同，并被转移到核周细胞质，在那里它逐渐解体。存在于相同细胞中的其他含肌动蛋白和黏着斑蛋白的结构，特别是基底处的局灶性接触，不受Ca 2+耗竭的类似影响，并保留与外表面的粘附以及与斑块和微丝组分的缔合。电子显微镜检查表明，与桥粒不同，粘附小带的膜结构域在Ca 2+去除后不被内吞，并且移位的含肌动蛋白和黏着斑蛋白的斑块和细丝带与特定的膜无关。它进一步表明，在恢复正常的Ca 2+水平的培养基中，新的细胞间的接触逐渐建立的粘着斑蛋白和肌动蛋白到新的带状斑块和microadhesive含有结构的增生。
EGTA-induced depletion of Ca2+ ions from the culture medium of Madin- Darby bovine kidney epithelial cells results in rapid splitting of adherens-type junctions and the detachment of the vinculin- and actin- containing filament bundle from the cytoplasmic faces of the plasma membrane of the zonula adhaerens. This process was monitored by phase- contrast microscopy, combined with electron microscopy and immunofluorescent localization of the two proteins. It is shown that shortly after extracellular free Ca2+ concentration is lowered to the micromolar range, the actin-containing, junction-associated belt of microfilaments, together with the vinculin-rich junctional plaque material, is irreversibly detached as one structural unit from the plasma membrane, contracts, and is displaced towards the perinuclear cytoplasm where it gradually disintegrates. Other actin- and vinculin- containing structures present in the same cells, notably the focal contacts at the substratum, are not similarly affected by the Ca2+ depletion and retain both the adhesion to the external surface and the association with the plaque and microfilament components. Electron microscopic examination has shown that the membrane domain of the zonulae adhaerentes, unlike that of desmosomes, is not endocytosed after Ca2+ removal and that the displaced actin- and vinculin- containing plaque and filament belt are not associated with a particular membrane. It is further shown that upon restoration of normal Ca2+ levels in the culture medium, new intercellular contacts are established gradually by accretion of both vinculin and actin into new belt-like plaque- and microfilament-containing structures.
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影响因子：
64.5
作者：
DAMSKY, CH;RICHA, J;BUCK, CA
通讯作者：
BUCK, CA
影响因子：
--
作者：
CEREIJIDO, M;MEZA, I;MARTINEZPALOMO, A
通讯作者：
MARTINEZPALOMO, A
DOI：
10.1083/jcb.100.1.327
发表时间：
1985-01
期刊：
The Journal of cell biology
影响因子：
--
作者：
Boller K;Vestweber D;Kemler R
通讯作者：
Kemler R
影响因子：
3.7
作者：
ABERCROMBIE, M;DUNN, GA
通讯作者：
DUNN, GA
DOI：
10.1083/jcb.20.2.199
发表时间：
1964-02
期刊：
The Journal of cell biology
影响因子：
--
作者：
CURTIS AS
通讯作者：
CURTIS AS