Overexpression of ethylene response factor TERF2 confers cold tolerance in rice seedlings

Overexpression of ethylene response factor TERF2 confers cold tolerance in rice seedlings
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乙烯反应因子TERF2的过表达赋予水稻幼苗耐寒性

DOI:
10.1007/s11248-010-9463-9
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发表时间:
2011-08
影响因子:
3
通讯作者:
Liu W
Liu W
中科院分区:
生物学4区
文献类型:
--
作者:
Chen X;Zhang H;Tian Y;Zhang Z;Huang R;Gao S;Lu X;Liu W

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水稻(Oryza sativa L.)是一种暖季植物,暴露在各种压力下。低温是限制水稻种植面积和产量扩大的重要因素。以前,我们已经证明番茄ERF蛋白TERF2增强转基因烟草和番茄植物的抗冻性。在此,我们报告TERF2的过表达增强转基因水稻的耐冷性,而不影响生长或农艺性状。结果表明,TERF 2不仅能增加低温胁迫下水稻体内渗透压物质和叶绿素的积累,还能降低活性氧(ROS)和丙二醛(MDA)含量,减少电解质渗漏。进一步的基因表达分析表明,TERF2基因在自然条件下或低温胁迫下能够激活OsMyb、OsICE 1、OsCDPK7、OsSODB、OsFer1、OsTrx23和OsLti6等低温相关基因的表达。因此,我们的研究结果表明,TERF2在低温胁迫下调控了胁迫相关基因的表达和一系列生理调节,表明TERF2在水稻响应非生物胁迫中可能具有重要的调控作用,在提高作物耐冷性方面具有潜在的应用价值。
Rice (Oryza sativa L.) is a warm-season plant exposed to various stresses. Low temperature is an important factor limiting extension of rice cultivation areas and productivity. Previously, we have demonstrated that tomato ERF protein TERF2 enhances freezing tolerance of transgenic tobacco and tomato plants. Herein, we report that overexpression of TERF2 enhances transgenic rice tolerance to cold without affecting growth or agronomic traits. Physiological assays revealed that TERF2 could not only increase accumulation of osmotic substances and chlorophyll, but also reduce reactive oxygen species (ROS) and malondialdehyde (MDA) content and decrease electrolyte leakage in rice under cold stress. Further analysis of gene expression showed that TERF2 could activate expression of cold-related genes, including OsMyb, OsICE1, OsCDPK7, OsSODB, OsFer1, OsTrx23, and OsLti6, in transgenic rice plants under natural condition or cold stress. Thus, our findings demonstrated that TERF2 modulated expression of stress-related genes and a series of physiological adjustments under cold stress, indicating that TERF2 might have important regulatory roles in response to abiotic stress in rice and possess potential utility in improving crop cold tolerance.
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发表时间: 2000-12-01
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影响因子: 7.4
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