The invasion and metastasis promotion role of CD97 small isoform in gastric carcinoma.
The invasion and metastasis promotion role of CD97 small isoform in gastric carcinoma.
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CD97小异构体在胃癌中的侵袭和转移促进作用
DOI：
10.1371/journal.pone.0039989
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发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Chen L
中科院分区：
文献类型：
--
作者：
Liu D;Trojanowicz B;Ye L;Li C;Zhang L;Li X;Li G;Zheng Y;Chen L
CD97在大多数胃腺癌中过表达，并与其去分化和侵袭性有关。我们之前的结果表明，在测试的三种CD97亚型中，只有较小的一种能够在体外促进增加的侵袭性。基于这些数据，我们进一步利用CD97小异构体稳定敲除的细胞和原位胃癌小鼠模型来研究CD97小异构体在体内胃癌进展中的作用。我们可以证明，与对照细胞相比，CD97/EGF1,2,5的敲低导致穿透明胶包被膜的细胞数量显著减少。在胃癌小鼠模型中，CD97/EGF1,2,5kd组和原位组均出现肿瘤肿块，且明显小于对照组。与SGC-NS组相比，CD97/EGF1、2,5kd组术后42天早期转移性区域淋巴结转移瘤细胞数量明显减少，并伴有CD44、VEGFR、CD31、CD97的下调。我们在本研究中得出结论，CD97小异构体不仅支持胃癌局部生长，而且促进原位植入小鼠模型的转移扩散，提示CD97小异构体参与（预）转移生态位的制备。
CD97 is over-expressed in the majority of gastric adenocarcinomas and is associated with its dedifferentiation and aggressiveness. Our previous results demonstrated that out of three CD97 isoforms tested, only the small one was able to promote increased invasiveness in vitro. Based on these data we further aimed to investigate the role of CD97 small isoform in gastric cancer progression in vivo by employing the cells with a stable CD97 small isoform knock-down and an orthotopic gastric cancer mouse model. We could demonstrate that the knock down of CD97/EGF1,2,5, led to a significant decrease in the number of cells penetrating the gelatin coated membrane as compared with control cells. In the gastric cancer mouse model, both the hypodermic and the orthotopic yielded tumor masses of the CD97/EGF1,2,5kd group and were significantly smaller than the control. Metastatic tumor cell number in early metastatic regional lymph nodes on post-operative day 42 was distinctly decreased in the CD97/EGF1,2,5kd group as compared with the SGC-NS group, and was accompanied with the downregulation of CD44, VEGFR, CD31 and CD97. We concluded in this study that CD97 small isoform not only supported gastric cancer local growth, but also promoted metastatic spread in orthotopically implanted mouse model suggesting involvement of the CD97 small isoform in the preparation of (pre)metastatic niche.
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影响因子：
4.3
作者：
Joyce, JA;Hanahan, D
通讯作者：
Hanahan, D
影响因子：
--
作者：
Jaspars, LH;Vos, W;Hamann, J
通讯作者：
Hamann, J
DOI：
10.1631/jzus.2005.b0913
发表时间：
2005-09-01
期刊：
Journal of Zhejiang University. Science. B
影响因子：
--
作者：
Liu, Yong;Chen, Li;Hoang-Vu, C
通讯作者：
Hoang-Vu, C
影响因子：
4.4
作者：
Hamann, J;Hartmann, E;vanLier, RAW
通讯作者：
vanLier, RAW
影响因子：
6.2
作者：
Nakanishi, Y;Ochiai, A;Hirohashi, S
通讯作者：
Hirohashi, S