Strain-specific colonization patterns and serum modulation of multi-species oral biofilm development.
Strain-specific colonization patterns and serum modulation of multi-species oral biofilm development.
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DOI：
10.1016/j.anaerobe.2012.06.003
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发表时间：
2012-08
期刊：
影响因子：
2.3
通讯作者：
Diaz, Patricia I.
中科院分区：
文献类型：
--
作者：
Biyikoglu, Basak;Ricker, Austin;Diaz, Patricia I.
关键词：
牙周炎是由龈下生物膜组成的生态变化引起的。龈下群落的成熟受到生物体间相互作用以及群落与宿主的关系的调节。为了更好地了解这一过程，我们评估了三种龈下流行微生物具核梭杆菌菌株和四种牙周病原牙龈卟啉单胞菌菌株与口腔共生物种的生物膜形成。我们还测试了类似于牙龈渗出物的血清对龈下生物膜的影响。使用唾液培养基在流动池中形成生物膜。我们发现，虽然并非所有具核梭菌菌株都能在单一物种生物膜中生长，但与健康相关的合作伙伴口放线菌和小韦荣球菌形成的群落促进了所有具核梭菌菌株的生物膜生长。牙龈卟啉单胞菌菌株也表现出形成单一物种生物膜的不同能力。牙龈卟啉单胞菌W50和W83没有形成生物膜，而ATCC 33277和381形成生物膜结构，但只有菌株ATCC 33277随时间生长。与具核梭菌与这两种健康相关物种的生长增强不同，牙龈卟啉单胞菌菌株在与口曲霉和小葡萄球菌的三物种群落中没有生长。然而，添加具核梭杆菌促进了牙龈卟啉单胞菌 ATCC 33277 与健康相关伙伴的生长。重要的是，血清对具核梭杆菌的粘附产生负面影响，同时有利于牙龈卟啉单胞菌生物膜的生长。这项工作强调了龈下生物膜形成中的菌株特异性。血清等环境因素会改变口腔微生物的定植模式，并可能通过选择性促进致病菌来影响龈下生物膜。
Periodontitis results from an ecological shift in the composition of subgingival biofilms. Subgingival community maturation is modulated by inter-organismal interactions and the relationship of communities with the host. In an effort to better understand this process, we evaluated biofilm formation, with oral commensal species, by three strains of the subgingivally prevalent microorganism Fusobacterium nucleatum and four strains of the periodontopathogen Porphyromonas gingivalis. We also tested the effect of serum, which resembles gingival exudates, on subgingival biofilms. Biofilms were allowed to develop in flow cells using salivary medium. We found that although not all strains of F. nucleatum were able to grow in mono-species biofilms, forming a community with health-associated partners Actinomyces oris and Veillonella parvula promoted biofilm growth of all F. nucleatum strains. Strains of P. gingivalis also showed variable ability to form mono-species biofilms. P. gingivalis W50 and W83 did not form biofilms, while ATCC 33277 and 381 formed biofilm structures, but only strain ATCC 33277 grew over time. Unlike the enhanced growth of F. nucleatum with the two health-associated species, no strain of P. gingivalis grew in three-species communities with A. oris and V. parvula. However, addition of F. nucleatum facilitated growth of P. gingivalis ATCC 33277 with health-associated partners. Importantly, serum negatively affected the adhesion of F. nucleatum, while it favored biofilm growth by P. gingivalis. This work highlights strain specificity in subgingival biofilm formation. Environmental factors such as serum alter the colonization patterns of oral microorganisms and could impact subgingival biofilms by selectively promoting pathogenic species.
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DOI：
10.1099/13500872-140-12-3407
发表时间：
1994-12-01
期刊：
MICROBIOLOGY-UK
影响因子：
--
作者：
BRADSHAW, DJ;HOMER, KA;BEIGHTON, D
通讯作者：
BEIGHTON, D
影响因子：
3.2
作者：
Chen, T;Hosogi, Y;Duncan, MJ
通讯作者：
Duncan, MJ
影响因子：
3.1
作者：
CISAR, JO;KOLENBRANDER, PE;MCINTIRE, FC
通讯作者：
MCINTIRE, FC
影响因子：
6.7
作者：
Jansen, HJ;vanderHoeven, JS
通讯作者：
vanderHoeven, JS
核细菌的基因组序列核亚种多形 - 一种遗传诱导的梭杆菌。
影响因子：
3.7
作者：
Karpathy, Sandor E.;Qin, Xiang;Gioia, Jason;Jiang, Huaiyang;Liu, Yamei;Petrosino, Joseph F.;Yerrapragada, Shailaja;Fox, George E.;Haake, Susan Kinder;Weinstock, George M.;Highlander, Sarah K.
通讯作者：
Highlander, Sarah K.