Genome sequence of Fusobacterium nucleatum subspecies polymorphum - a genetically tractable fusobacterium.
Genome sequence of Fusobacterium nucleatum subspecies polymorphum - a genetically tractable fusobacterium.
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核细菌的基因组序列核亚种多形 - 一种遗传诱导的梭杆菌。
DOI：
10.1371/journal.pone.0000659
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发表时间：
2007-08-01
期刊：
影响因子：
3.7
通讯作者：
Highlander, Sarah K.
中科院分区：
文献类型：
--
作者：
Karpathy, Sandor E.;Qin, Xiang;Gioia, Jason;Jiang, Huaiyang;Liu, Yamei;Petrosino, Joseph F.;Yerrapragada, Shailaja;Fox, George E.;Haake, Susan Kinder;Weinstock, George M.;Highlander, Sarah K.
具核梭杆菌是口腔微生物群的重要成员，也是人类感染的常见原因。 F. nucleatum 包括五个亚种：polymorphum、nucleatum、vincentii、fusiforme 和animalis。 F. nucleatum subsp.多态性 ATCC 10953 的表型已得到很好的表征，与之前测序的菌株相比，它适合基因转移。我们对 F. nucleatum subsp. 的 2,429,698 bp 基因组进行了测序和注释。多态性 ATCC 10953。还对来自该菌株的质粒 pFN3 进行了测序和分析。与其他两个可用的梭杆菌基因组（F. nucleatum subsp. nucleatum 和 F. nucleatum subsp. vincentii）相比，F. nucleatum subsp. vincentii 特有的 627 个开放阅读框。鉴定出多晶型物ATCC 10953。其中很大一部分位于包含 5 个或更多基因的 28 个区域或岛屿之一。表现出相似性的簇状蛋白质中，有 17% 与来自梭状芽胞杆菌的蛋白质最相似，其他则与来自其他革兰氏阳性生物（如芽孢杆菌和链球菌）的蛋白质最相似。鉴定出与鼠伤寒沙门氏菌丙二醇利用位点同源的十个碱基区域，以及原噬菌体和整合的接合质粒。该基因组包含五个复合核酶/转座子，类似于艰难梭菌中描述的 CdISt IStron。 IStron 不存在于其他梭杆菌基因组中。这些发现表明 F. nucleatum subsp.多形菌擅长水平基因转移，并且与厚壁菌门，特别是梭状芽胞杆菌的交换很常见。
Fusobacterium nucleatum is a prominent member of the oral microbiota and is a common cause of human infection. F. nucleatum includes five subspecies: polymorphum, nucleatum, vincentii, fusiforme, and animalis. F. nucleatum subsp. polymorphum ATCC 10953 has been well characterized phenotypically and, in contrast to previously sequenced strains, is amenable to gene transfer. We sequenced and annotated the 2,429,698 bp genome of F. nucleatum subsp. polymorphum ATCC 10953. Plasmid pFN3 from the strain was also sequenced and analyzed. When compared to the other two available fusobacterial genomes (F. nucleatum subsp. nucleatum, and F. nucleatum subsp. vincentii) 627 open reading frames unique to F. nucleatum subsp. polymorphum ATCC 10953 were identified. A large percentage of these mapped within one of 28 regions or islands containing five or more genes. Seventeen percent of the clustered proteins that demonstrated similarity were most similar to proteins from the clostridia, with others being most similar to proteins from other gram-positive organisms such as Bacillus and Streptococcus. A ten kilobase region homologous to the Salmonella typhimurium propanediol utilization locus was identified, as was a prophage and integrated conjugal plasmid. The genome contains five composite ribozyme/transposons, similar to the CdISt IStrons described in Clostridium difficile. IStrons are not present in the other fusobacterial genomes. These findings indicate that F. nucleatum subsp. polymorphum is proficient at horizontal gene transfer and that exchange with the Firmicutes, particularly the Clostridia, is common.
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DOI：
10.1073/pnas.0335853100
发表时间：
2003-02-04
影响因子：
11.1
作者：
Brüggemann, H;Bäumer, S;Gottschalk, G
通讯作者：
Gottschalk, G
影响因子：
11.8
作者：
CIVEN, R;JOUSIMIESSOMER, H;FINEGOLD, SM
通讯作者：
FINEGOLD, SM
DOI：
10.1111/j.1471-0528.2002.01349.x
发表时间：
2002-05-01
影响因子：
5.8
作者：
Bearfield, C;Davenport, ES;Allaker, RP
通讯作者：
Allaker, RP
影响因子：
3.2
作者：
Bobik, TA;Havemann, GD;Aldrich, HC
通讯作者：
Aldrich, HC
影响因子：
3
作者：
Brook, I;Frazier, EH
通讯作者：
Frazier, EH