Assessment of circadian rhythms in humans: comparison of real-time fibroblast reporter imaging with plasma melatonin.-猫眼课题宝

人类昼夜节律的评估：实时成纤维细胞报告成像与血浆褪黑激素的比较。

DOI：

10.1096/fj.11-201699

发表时间：

2012-06

期刊：

FASEB journal : official publication of the Federation of American Societies for Experimental Biology

影响因子：

通讯作者：

中科院分区：

其他

文献类型：

作者：

Hasan S;Santhi N;Lazar AS;Slak A;Lo J;von Schantz M;Archer SN;Johnston JD;Dijk DJ

来源链接：

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3360144/?report=classic

我们比较了由下丘脑昼夜节律起搏器驱动的血浆褪黑激素的节律周期与体外培养的外周成纤维细胞的周期性，以评估与睡眠时间相关的PER3多态性（rs57875989）对这些节律的影响。荧光素酶的表达是由昼夜节律基因Arntl （Bmal1）的启动子驱动的。褪黑激素节律周期在9 d强制非同步方案中进行评估，最大限度地减少睡眠-觉醒和光-暗周期对昼夜节律的混淆影响。体外周期（32名参与者，24.61±0.33小时，平均±sd）比体内周期（31名参与者，24.16±0.17小时，P<0.0001）更长，但PER3基因型之间没有差异（P < 0.4）。重复体外评估分析表明，昼夜节律在个体内是可重复的（类内相关=0.62），但体内和体外周期评估不相关（P < 0.9）。根据昼夜节律干扰理论，体内周期与基线时褪黑激素的使用时间相关（P<0.05），与昼夜偏好相关（P<0.05）。个体昼夜节律可以在成纤维细胞中可靠地评估，但可能与中央昼夜节律起搏器驱动的生理节律无关。-Hasan， S., Santhi， N., Lazar, a.s., Slak， A., Lo， J., von Schantz， M., Archer， S. N., Johnston， J. D., Dijk， D.-J.。人类昼夜节律的评估：实时成纤维细胞报告成像与血浆褪黑素的比较。

We compared the period of the rhythm of plasma melatonin, driven by the hypothalamic circadian pacemaker, to in vitro periodicity in cultured peripheral fibroblasts to assess the effects on these rhythms of a polymorphism of PER3 (rs57875989), which is associated with sleep timing. In vitro circadian period was determined using luminometry of cultured fibroblasts, in which the expression of firefly luciferase was driven by the promoter of the circadian gene Arntl (Bmal1). The period of the melatonin rhythm was assessed in a 9-d forced desynchrony protocol, minimizing confounding effects of sleep-wake and light-dark cycles on circadian rhythmicity. In vitro periods (32 participants, 24.61±0.33 h, mean±sd) were longer than in vivo periods (31 participants, 24.16±0.17 h; P<0.0001) but did not differ between PER3 genotypes (P>0.4). Analyses of replicate in vitro assessments demonstrated that circadian period was reproducible within individuals (intraclass correlation=0.62), but in vivo and in vitro period assessments did not correlate (P>0.9). In accordance with circadian entrainment theory, in vivo period correlated with the timing of melatonin (P<0.05) at baseline and with diurnal preference (P<0.05). Individual circadian rhythms can be reliably assessed in fibroblasts but may not correlate with physiological rhythms driven by the central circadian pacemaker.—Hasan, S., Santhi, N., Lazar, A.S., Slak, A., Lo, J., von Schantz, M., Archer, S. N., Johnston, J. D., Dijk, D.-J. Assessment of circadian rhythms in humans: comparison of real-time fibroblast reporter imaging with plasma melatonin.