Mycobacterium tuberculosis infection in rhesus monkeys (Macaca mulatta) and evaluation of ESAT-6 and CFP10 as immunodiagnostic antigens.
Mycobacterium tuberculosis infection in rhesus monkeys (Macaca mulatta) and evaluation of ESAT-6 and CFP10 as immunodiagnostic antigens.
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DOI：
10.1538/expanim.62.281
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发表时间：
2013
影响因子：
2.4
通讯作者：
Yuan W
中科院分区：
文献类型：
--
作者：
Min F;Zhang Y;Pan J;Wang J;Yuan W
非人灵长类动物的结核病（TB）对与它们接触的动物和人类的福祉构成严重威胁，而快速、准确和可靠的诊断具有挑战性。在这项研究中，我们首先试图建立一个合适的灵长类动物结核病模型，类似于自然结核病在非人灵长类动物。用两种低剂量的M.结核病H37 Rv.无论感染剂量如何，通过临床评估、结核菌素皮肤试验转换、外周免疫应答、大体观察、组织病理学分析和M.结核病负担。此外，我们扩展了该模型用于评估以下免疫诊断抗原的有用性：CFP 10、ESAT-6、CFP 10-ESAT-6以及CFP 10和ESAT-6的抗原混合物。结果表明，CFP 10为M.结核病特异性“早期”抗原，用于非人灵长类动物结核病的血清学诊断。本研究建立了一种基于低剂量结核分枝杆菌的灵长类动物结核病模型，为结核分枝杆菌的研究提供了新的契机。结核病和诊断。
Tuberculosis (TB) in nonhuman primates is a serious menace to the welfare of the animals and human who come into contact with them, while the rapid, accurate, and robust diagnosis is challenging. In this study, we first sought to establish an appropriate primate TB model resembling natural TB in nonhuman primates. Four rhesus monkeys (Macaca mulatta) of Chinese origin were infected intratracheally with two low doses of M. tuberculosis H37Rv. Regardless of the infectious doses, all monkeys were demonstrated to be successfully infected by clinical assessments, tuberculin skin test conversions, peripheral immune responses, gross observations, histopathology analysis, and M. tuberculosis burdens. Furthermore, we extended the usefulness of this model for assessing the following immunodiagnostic antigens: CFP10, ESAT-6, CFP10-ESAT-6, and an antigen cocktail of CFP10 and ESAT-6. The data showed that CFP10 was an M. tuberculosis-specific, “early” antigen used for serodiagnosis of TB in nonhuman primates. In conclusion, we established a useful primate TB model depending on low doses of M .tuberculosis and affording new opportunities for studies of M. tuberculosis disease and diagnostics.
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影响因子：
6.4
作者：
Kunnath-Velayudhan, Shajo;Davidow, Amy L.;Gennaro, Maria L.
通讯作者：
Gennaro, Maria L.
影响因子：
0.7
作者：
Gormus, BJ;Blanchard, JL;Didier, PJ
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影响因子：
82.9
作者：
Walsh, GP;Tan, EV;Horwitz, MA
通讯作者：
Horwitz, MA
DOI：
10.1073/pnas.1130426100
发表时间：
2003-06-24
影响因子：
11.1
作者：
Garnier, T;Eiglmeier, K;Hewinson, RG
通讯作者：
Hewinson, RG
影响因子：
64.8
作者：
Cole, ST;Brosch, R;Barrell, BG
通讯作者：
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