Isolation and characterization of methicillin-resistant Staphylococcus aureus from pork farms and visiting veterinary students.
Isolation and characterization of methicillin-resistant Staphylococcus aureus from pork farms and visiting veterinary students.
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从猪肉农场和访问兽医的学生中的耐甲氧西林金黄色葡萄球菌的隔离和表征。
DOI：
10.1371/journal.pone.0053738
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Smith TC
中科院分区：
文献类型：
--
作者：
Frana TS;Beahm AR;Hanson BM;Kinyon JM;Layman LL;Karriker LA;Ramirez A;Smith TC
在过去的十年中，家畜相关的耐甲氧西林S。金黄色葡萄球菌（LA-MRSA）已成为世界许多地方的公共卫生问题。序列型398（ST 398）是最常报告的LA-MRSA类型。虽然许多研究都集中在养猪工人经历的长期暴露，本研究的重点是兽医学生进行诊断调查经历的短期暴露。目的是评估暴露于养猪场的学生MRSA获得率和携带寿命，并描述回收的MRSA分离株。在农场访问前后立即收集学生鼻拭子。还收集了猪鼻拭子和环境海绵样品。MRSA分离株进行了生化和分子鉴定，包括spa分型和抗菌药物敏感性测试。招收了三十（30）名兽医学生，访问了40个养猪场。在30%的养猪场和22%的学生中检测到MRSA，这些学生在接触MRSA阳性的养猪场后。所有在农场参观后最初发现MRSA阳性的学生在参观后24小时内对MRSA呈阴性。最常见的spa类型是t002（79%），t034（16%）和t548（4%）。在猪肉养殖场发现的温泉类型与从学生身上发现的温泉类型几乎一致。对抗生素的耐药水平各不相同，但最常见的耐药是壮观霉素、四环素和新霉素。与ST 398 MRSA分离株相比，非ST 398 MRSA分离株更可能对氟苯尼考和新霉素耐药，并且更可能具有多重耐药。这些发现表明，MRSA可以从访问受污染农场的人身上回收。然而，携带的持续时间非常短，最有可能代表鼻腔污染，而不是生物定植。在这项研究中发现的最常见的温泉类型与ST 5相关，并扩大了牲畜相关MRSA类型的范围。
In the last decade livestock-associated methicillin-resistant S. aureus (LA-MRSA) has become a public health concern in many parts of the world. Sequence type 398 (ST398) has been the most commonly reported type of LA-MRSA. While many studies have focused on long-term exposure experienced by swine workers, this study focuses on short-term exposures experienced by veterinary students conducting diagnostic investigations. The objectives were to assess the rate of MRSA acquisition and longevity of carriage in students exposed to pork farms and characterize the recovered MRSA isolates. Student nasal swabs were collected immediately before and after farm visits. Pig nasal swabs and environmental sponge samples were also collected. MRSA isolates were identified biochemically and molecularly including spa typing and antimicrobial susceptibility testing. Thirty (30) veterinary students were enrolled and 40 pork farms were visited. MRSA was detected in 30% of the pork farms and in 22% of the students following an exposure to a MRSA-positive pork farm. All students found to be MRSA-positive initially following farm visit were negative for MRSA within 24 hours post visit. Most common spa types recovered were t002 (79%), t034 (16%) and t548 (4%). Spa types found in pork farms closely matched those recovered from students with few exceptions. Resistance levels to antimicrobials varied, but resistance was most commonly seen for spectinomycin, tetracyclines and neomycin. Non-ST398 MRSA isolates were more likely to be resistant to florfenicol and neomycin as well as more likely to be multidrug resistant compared to ST398 MRSA isolates. These findings indicate that MRSA can be recovered from persons visiting contaminated farms. However, the duration of carriage was very brief and most likely represents contamination of nasal passages rather than biological colonization. The most common spa types found in this study were associated with ST5 and expands the range of livestock-associated MRSA types.
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影响因子：
3.9
作者：
Bootsma, Martin C. J.;Wassenberg, Marjan W. M.;Bonten, Marc J. M.
通讯作者：
Bonten, Marc J. M.
影响因子：
2.2
作者：
Dressler, A. E.;Scheibel, R. P.;Smith, T. C.
通讯作者：
Smith, T. C.
影响因子：
3.3
作者：
Khanna, T.;Friendship, R.;Weese, J. S.
通讯作者：
Weese, J. S.
影响因子：
3.3
作者：
de Neeling, A. J.;van den Broek, M. J. M.;Huijsdens, X. W.
通讯作者：
Huijsdens, X. W.
影响因子：
3.7
作者：
Cuny C;Nathaus R;Layer F;Strommenger B;Altmann D;Witte W
通讯作者：
Witte W