Nasal colonization of humans with methicillin-resistant Staphylococcus aureus (MRSA) CC398 with and without exposure to pigs.
Nasal colonization of humans with methicillin-resistant Staphylococcus aureus (MRSA) CC398 with and without exposure to pigs.
复制标题
DOI：
10.1371/journal.pone.0006800
复制
发表时间：
2009-08-27
期刊：
影响因子：
3.7
通讯作者：
Witte W
中科院分区：
文献类型：
--
作者：
Cuny C;Nathaus R;Layer F;Strommenger B;Altmann D;Witte W
在几个欧洲国家和北美的研究表明，MRSA CC398经常对牲畜和与被殖民动物有直接职业接触的人进行鼻腔定殖化。这里介绍的这项研究解决了进一步传播给未接触病毒的人的问题。在选择了德国不同地区的47个养猪场后，我们对113名每天与猪一起工作的人及其116名未接触过猪的家庭成员的鼻孔进行了采样。参加养猪场的18名兽医及其44名未接触病毒的家庭成员也进行了同样的测试。为了调查家庭以外的传播，我们对高密度养猪区一所中学的462名学生的鼻孔进行了抽样。用选择性琼脂平板直接培养法检测MRSA。对分离株进行SpA-Sequence分型和SCCmec基因分型。对于SpA序列类型与多位点序列分型所定义的克隆谱系的归属，我们使用了Burp算法。药敏试验采用微量肉汤稀释法。在被调查的农场中，86%的人暴露在空气中，他们的家庭成员中只有4.3%的人被发现携带MRSA，表现出与克隆复合体CC398相对应的spa类型。在照料养猪场的兽医中也发现了鼻腔定植，在他们未接触过的家庭成员中也发现了9%的鼻部定植。多变量分析显示，在采样前使用抗生素与定植没有风险。在462名学生中，只有3人被发现是殖民地，所有3人都生活在养猪场。这些结果表明，到目前为止，MRSA CC398在非暴露人群中的传播很少见，可能不会超出家庭社区的范围。
Studies in several European countries and in North America revealed a frequent nasal colonization of livestock with MRSA CC398 and also in humans with direct professional exposure to colonized animals. The study presented here addresses the question of further transmission to non exposed humans. After selecting 47 farms with colonized pigs in different regions of Germany we sampled the nares of 113 humans working daily with pigs and of their 116 non exposed family members. The same was performed in 18 veterinarians attending pig farms and in 44 of their non exposed family members. For investigating transmission beyond families we samples the nares of 462 pupils attending a secondary school in a high density pig farming area. MRSA were detected by direct culture on selective agar. The isolates were typed by means of spa-sequence typing and classification of SCCmec elements. For attribution of spa sequence types to clonal lineages as defined by multi locus sequence typing we used the BURP algorithm. Antibiotic susceptibility testing was performed by microbroth dilution assay. At the farms investigated 86% of humans exposed and only 4.3% of their family members were found to carry MRSA exhibiting spa-types corresponding to clonal complex CC398. Nasal colonization was also found in 45% of veterinarians caring for pig farms and in 9% of their non exposed family members. Multivariate analysis revealed that antibiotic usage prior to sampling beard no risk with respect to colonization. From 462 pupils only 3 were found colonized, all 3 were living on pig farms. These results indicate that so far the dissemination of MRSA CC398 to non exposed humans is infrequent and probably does not reach beyond familial communities.
登录
查看更多内容
影响因子：
3
作者：
Sergio, Darvi Michell Bufete;Koh, Tse Hsien;Chow, Pierce K. H.
通讯作者：
Chow, Pierce K. H.
影响因子：
3.3
作者：
Khanna, T.;Friendship, R.;Weese, J. S.
通讯作者：
Weese, J. S.
影响因子：
3.3
作者：
de Neeling, A. J.;van den Broek, M. J. M.;Huijsdens, X. W.
通讯作者：
Huijsdens, X. W.
影响因子：
4.4
作者：
Mainous, AG;Hueston, WJ;Diaz, VA
通讯作者：
Diaz, VA
影响因子：
11.8
作者：
Tiemersma EW;Bronzwaer SL;Lyytikäinen O;Degener JE;Schrijnemakers P;Bruinsma N;Monen J;Witte W;Grundman H;European Antimicrobial Resistance Surveillance System Participants
通讯作者：
European Antimicrobial Resistance Surveillance System Participants