Metabolic Profiling of Human Blood by High Resolution Ion Mobility Mass Spectrometry (IM-MS).
Metabolic Profiling of Human Blood by High Resolution Ion Mobility Mass Spectrometry (IM-MS).
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DOI：
10.1016/j.ijms.2010.02.007
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发表时间：
2010-12
影响因子：
1.8
通讯作者：
Hill HH
中科院分区：
文献类型：
--
作者：
Dwivedi P;Schultz AJ;Hill HH
评价了具有电喷雾电离源的高分辨率离子迁移率飞行时间质谱仪（ESI-IM-MS）作为快速分析人体血液代谢组等复杂生物样品的分析方法。混合仪器（IM-MS）提供的平均离子迁移率分辨率约为90，质量分辨率约为1500（m/z 100）。用甲醇提取几微升全血，离心并通过电喷雾电离源注入IM-MS。在对人血液代谢物组进行IM-MS分析后，在短分析时间（30分钟）内检测到约1，100种代谢物离子，并分离出300种异构代谢物。代谢物的估计浓度范围为低微摩尔至低纳摩尔水平。发现各种代谢物（氨基酸、有机酸、脂肪酸、碳水化合物、嘌呤和嘧啶等）形成特征迁移率-质量相关曲线（MMCC），有助于代谢物鉴定。对应于各种甾醇衍生物，雌激素衍生物，磷酸胆碱，三尖杉酯碱，和胆固醇衍生物中检测到的血液提取物的峰被发现占据特征的二维IM-MS空间。通过在迁移率空间中进行区分，将MS随机噪声中可能丢失的低丰度代谢物峰与噪声峰分离。此外，通过在MS分析之前偶联IMS，MS的峰容量增加了6倍。
A high resolution ion mobility time-of-flight mass spectrometer with electrospray ionization source (ESI-IM-MS) was evaluated as an analytical method for rapid analysis of complex biological samples such as human blood metabolome was investigated. The hybrid instrument (IM-MS) provided an average ion mobility resolving power of ~90 and a mass resolution of ~1500 (at m/z 100). A few µL of whole blood was extracted with methanol, centrifuged and infused into the IM-MS via an electrospray ionization source. Upon IM-MS profiling of the human blood metabolome approximately 1,100 metabolite ions were detected and 300 isomeric metabolites separated in short analyses time (30 minutes). Estimated concentration of the metabolites ranged from the low micromolar to the low nanomolar level. Various classes of metabolites (amino acids, organic acids, fatty acids, carbohydrates, purines and pyrimidines etc) were found to form characteristic mobility-mass correlation curves (MMCC) that aided in metabolite identification. Peaks corresponding to various sterol derivatives, estrogen derivatives, phosphocholines, prostaglandins, and cholesterol derivatives detected in the blood extract were found to occupy characteristic two dimensional IM-MS space. Low abundance metabolite peaks that can be lost in MS random noise were resolved from noise peaks by differentiation in mobility space. In addition, the peak capacity of MS increased six fold by coupling IMS prior to MS analysis.
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影响因子：
13.1
作者：
Dunn, WB;Ellis, DI
通讯作者：
Ellis, DI
DOI：
10.1007/s12127-008-0006-5
发表时间：
2008-12-01
影响因子：
--
作者：
Dwivedi, Prabha;Hill, Herbert H., Jr.
通讯作者：
Hill, Herbert H., Jr.
DOI：
10.1016/j.jpba.2008.01.028
发表时间：
2008-06-09
影响因子：
3.4
作者：
Barbas, C.;Vallejo, M.;Hanna-Brown, A.
通讯作者：
Hanna-Brown, A.
DOI：
10.1016/j.jasms.2007.04.007
发表时间：
2007-07-01
影响因子：
3.2
作者：
Dwivedi, Prabha;Bendiak, Brad;Hill, Herbert H., Jr.
通讯作者：
Hill, Herbert H., Jr.
影响因子：
7.4
作者：
EICEMAN, GA;BLYTH, DA;SNYDER, AP
通讯作者：
SNYDER, AP