The effect of source animal age upon extracellular matrix scaffold properties.
The effect of source animal age upon extracellular matrix scaffold properties.
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DOI：
10.1016/j.biomaterials.2010.09.006
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发表时间：
2011-01
期刊：
影响因子：
14
通讯作者：
Badylak, Stephen F.
中科院分区：
文献类型：
--
作者：
Tottey, Stephen;Johnson, Scott A.;Crapo, Peter M.;Reing, Janet E.;Zhang, Li;Jiang, Hongbin;Medberry, Christopher J.;Reines, Brandon;Badylak, Stephen F.
由哺乳动物细胞外基质（ECM）组成的生物支架材料通常用于损伤组织的修复和重建。生物支架的一个重要但尚未探索的变量是制备 ECM 的动物的年龄。本研究的目的是比较从仅年龄不同的猪身上采集的小肠粘膜下层 (SIS)-ECM 的结构、机械和成分特性。还检查了这些 ECM 材料的降解产物生物活性。结果表明，不同年龄来源的 ECM 支架之间的每个变量都存在明显差异。来自 3 周龄动物的 ECM 的强度和生长因子含量低于从 12、26 或 > 52 周龄动物收获的 ECM。 3周和>52周源的SIS-ECM弹性模量小于12周和26周源的弹性模量。所有年龄来源的 ECM 的降解产物都对血管周围干细胞具有趋化作用，其中 12 周的来源最有效，而最古老的来源引起的增殖增加最大。总之，从不同年龄的动物中获取的 SIS-ECM 的机械、结构和生物学特性存在明显差异。
Biologic scaffold materials composed of mammalian extracellular matrix (ECM) are commonly used for the repair and reconstruction of injured tissues. An important, but unexplored variable of biologic scaffolds is the age of the animal from which the ECM is prepared. The objective of the present study was to compare the structural, mechanical, and compositional properties of small intestinal submucosa (SIS)-ECM harvested from pigs that differed only in age. Degradation product bioactivity of these ECM materials was also examined. Results showed that there are distinct differences in each of these variables among the various age source ECM scaffolds. The strength and growth factors content of ECM from 3 week old animals is less than that of ECM harvested from 12, 26 or >52 week old animals. The elastic modulus of SIS-ECM for 3 week and >52 week old source was less than that of the 12 and 26 week source. Degradation products from all age source ECMs were chemotactic for perivascular stem cells, with the 12 week source the most potent, while the oldest source caused the greatest increase in proliferation. In summary, distinct differences exist in the mechanical, structural, and biologic properties of SIS-ECM harvested from different aged animals.
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影响因子：
14
作者：
Brown, Bryan N.;Barnes, Christopher A.;Kasick, Rena T.;Michel, Roger;Gilbert, Thomas W.;Beer-Stolz, Donna;Castner, David G.;Ratner, BuddyD.;Badylak, Stephen F.
通讯作者：
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影响因子：
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作者：
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通讯作者：
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