Biotechnological advances in the diagnosis, species differentiation and phylogenetic analysis of Schistosoma spp.
Biotechnological advances in the diagnosis, species differentiation and phylogenetic analysis of Schistosoma spp.
复制标题
血吸虫诊断、物种分化和系统发育分析的生物技术进展。
DOI：
10.1016/j.biotechadv.2012.02.008
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发表时间：
2012-11
影响因子：
16
通讯作者：
Xing-Quan Zhu
中科院分区：
文献类型：
--
作者：
Guang-Hui Zhao;Juan Li;David Blair;Xiao-Yan Li;Hany M. Elsheikha;Rui-Qing Lin;Feng-Cai Zou;Xing-Quan Zhu
血吸虫病是一种严重的寄生虫病，由血吸虫属的血吸虫引起。在全世界，血吸虫病的发病率和死亡率都很高，有近8亿人面临感染风险。血吸虫种类鉴定和血吸虫病诊断的精确方法对于加强对寄生虫流行病学的理解至关重要，从而为有效的抗寄生虫治疗和预防措施提供信息。血吸虫病的传统诊断方法包括病原学、免疫学和影像学技术。血吸虫病的诊断已被革命性的新分子技术的出现，以扩增寄生虫核酸。其中，聚合酶链反应为基础的方法已在血吸虫属之间的遗传变异的分析是有用的。质谱法现在正在扩大可以检测的生物分子的范围。本文综述了传统的非DNA诊断方法，并对血吸虫的诊断、种间鉴别和系统发育分析的分子生物学技术进行了描述和讨论。这些激动人心的技术为进一步发展更有效、更精确的方法来区分寄生虫和临床诊断血吸虫病提供了基础，也对探索新的控制血吸虫病的措施具有重要意义。
Schistosomiasis is a serious parasitic disease caused by blood-dwelling flukes of the genus Schistosoma. Throughout the world, schistosomiasis is associated with high rates of morbidity and mortality, with close to 800million people at risk of infection. Precise methods for identification of Schistosoma species and diagnosis of schistosomiasis are crucial for an enhanced understanding of parasite epidemiology that informs effective antiparasitic treatment and preventive measures. Traditional approaches for the diagnosis of schistosomiasis include etiological, immunological and imaging techniques. Diagnosis of schistosomiasis has been revolutionized by the advent of new molecular technologies to amplify parasite nucleic acids. Among these, polymerase chain reaction-based methods have been useful in the analysis of genetic variation among Schistosoma spp. Mass spectrometry is now extending the range of biological molecules that can be detected. In this review, we summarize traditional, non-DNA-based diagnostic methods and then describe and discuss the current and developing molecular techniques for the diagnosis, species differentiation and phylogenetic analysis of Schistosoma spp. These exciting techniques provide foundations for further development of more effective and precise approaches to differentiate schistosomes and diagnose schistosomiasis in the clinic, and also have important implication for exploring novel measures to control schistosomiasis in the near future.
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影响因子：
3.8
作者：
Jones, Malcolm K.;Balen, Julie
通讯作者：
Balen, Julie
DOI：
10.1016/j.trstmh.2007.09.017
发表时间：
2008
影响因子：
2.2
作者：
M. L. M. Robijn-M.-L.-M.-Robijn-13696999;J. Planken;D. Kornelis;C. Hokke;A. Deelder
通讯作者：
M. L. M. Robijn-M.-L.-M.-Robijn-13696999;J. Planken;D. Kornelis;C. Hokke;A. Deelder
影响因子：
2.7
作者：
Agola LE;Steinauer ML;Mburu DN;Mungai BN;Mwangi IN;Magoma GN;Loker ES;Mkoji GM
通讯作者：
Mkoji GM
影响因子：
--
作者：
A. Simpson;S. Smithers
通讯作者：
A. Simpson;S. Smithers
影响因子：
3.8
作者：
Rudge JW;Carabin H;Balolong E;Tallo V;Shrivastava J;Lu DB;Basáñez MG;Olveda R;McGarvey ST;Webster JP
通讯作者：
Webster JP