The Regulatory Mechanism of MLT/MT1 Signaling on the Growth of Antler Mesenchymal Cells.
The Regulatory Mechanism of MLT/MT1 Signaling on the Growth of Antler Mesenchymal Cells.
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MLT/MT1信号对鹿茸间充质细胞生长的调控机制
DOI：
10.3390/molecules22101793
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发表时间：
2017-10-23
期刊：
影响因子：
--
通讯作者：
Huo L
中科院分区：
文献类型：
--
作者：
Yang F;He C;Sun X;Wang J;Luo C;Liu G;Yang L;Xiong J;Huo L
褪黑激素（Melatonin，MLT）在调节季节性繁殖动物的生理周期中起着重要作用。褪黑素受体I（MT 1）在鹿茸形成层中有效表达。然而，MLT/MT 1信号在西卡间充质细胞中的功能和代谢机制仍有待进一步阐明。本研究旨在探讨MLT/MT 1信号通路对间充质细胞增殖的影响以及MLT/MT 1与IGF 1/IGF 1-R信号通路的相互作用。结果表明：（1）鹿茸间充质细胞实际上表达MT 1，（2）外源性褪黑素显著促进鹿茸间充质细胞增殖，而MT 1敲低则显著削弱褪黑素的正效应;（3）褪黑激素显著增强IGF 1/IGF 1-R信号传导，因为IGF 1和IGF-1 R的表达均增加，而MT 1敲低显著降低IGF 1-R表达和IGF 1合成。综上所述，这些数据证实了MLT/MT 1信号在鹿茸间充质增殖中起着至关重要的作用，这可能是由IGF 1/IGF 1-R介导的。
Melatonin (MLT) plays an important role in regulating the physiological cycle of seasonal breeding animals. Melatonin receptor I (MT1) is effectively expressed in the cambium layer of deer antler. However, the function and metabolic mechanism of MLT/MT1 signaling in the mesenchymal cells of sika deer remain to be further elucidated. In this work, we detected the effects of MLT/MT1 signaling on mesenchymal cells proliferation and the interaction between MLT/MT1 and IGF1/IGF1-R signaling. The results show that (1) deer antler mesenchymal cells actually express MT1; (2) exogenous melatonin significantly promotes mesenchymal cells proliferation, while MT1 knock-down significantly impairs the positive effects of melatonin; and (3) melatonin significantly enhanced IGF1/IGF1-R signaling, as both the expression of IGF1 and IGF-1R increased, while MT1 knock-down significantly decreased IGF1-R expression and IGF1 synthesis. In summary, these data verified that MLT/MT1 signaling plays a crucial role in antler mesenchymal proliferation, which may be mediated by IGF1/IGF1-R.
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影响因子：
5.6
作者：
He C;Wang J;Zhang Z;Yang M;Li Y;Tian X;Ma T;Tao J;Zhu K;Song Y;Ji P;Liu G
通讯作者：
Liu G
DOI：
10.1002/ar.a.20127
发表时间：
2004-12-01
期刊：
ANATOMICAL RECORD PART A-DISCOVERIES IN MOLECULAR CELLULAR AND EVOLUTIONARY BIOLOGY
影响因子：
--
作者：
Kierdorf, U;Kierdorf, H;Rolf, HJ
通讯作者：
Rolf, HJ
影响因子：
10.3
作者：
BUBENIK, GA;SMITH, PS;SCHAMS, D
通讯作者：
SCHAMS, D
影响因子：
0.6
作者：
Marková, M;Adámeková, E;Ahlers, I
通讯作者：
Ahlers, I
妊娠早期小鼠子宫中表达的褪黑激素相关基因促进胚胎着床
影响因子：
10.3
作者：
He, Changjiu;Wang, Jing;Liu, Guoshi
通讯作者：
Liu, Guoshi