Increasing lysine content of waxy maize through introgression of opaque-2 and opaque-16 genes using molecular assisted and biochemical development.
Increasing lysine content of waxy maize through introgression of opaque-2 and opaque-16 genes using molecular assisted and biochemical development.
复制标题
利用分子辅助和生化开发通过 Opaque-2 和 Opaque-16 基因渗入来提高糯玉米的赖氨酸含量
DOI：
10.1371/journal.pone.0056227
复制
发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Cai Y
中科院分区：
文献类型：
--
作者：
Zhang W;Yang W;Wang M;Wang W;Zeng G;Chen Z;Cai Y
糯玉米的赖氨酸含量低，不能满足人、牲畜或家禽的营养需求。本研究以玉米高赖氨酸自交系台系19（o2 o2）和QCL 3021（o 16 o 16）为供体，糯玉米自交系QCL 5019（wxwx）为受体，将o2和o 16基因回交到wx系中。在三交F1、回交和自交世代中，利用wx和o2基因内的SSR标记phi 027和phi 112以及与o 16基因连锁的SSR标记umc 1121进行前景选择。对所选个体进行全基因组SSR标记的背景选择。采用染料结合赖氨酸法测定籽粒赖氨酸含量。采用碘-碘化钾染色法和双波长分光光度法测定籽粒蜡质。BC 2F 2代包括基因型wxwxo 2 o2o 16 o 16_的7个植株、基因型wxwxo16o16O2_的19个植株和基因型wxwxo 2 o2o 16 o 16的3个植株。在这些种子中，平均支链淀粉含量分别为96.67%、96.87%和96.62%，这与QCL 5019相似。平均赖氨酸含量分别为0.555%、0.380%和0.616%，分别比QCL 5019提高75.1%、19.9%和94.3%。BC_2F_3家系的平均遗传背景恢复率分别为95.3%、94.3%、94.2%。其中3个wxwxo 2 o2o 16 o 16家系、4个wxwxo 2 o2o 16 o 16家系和3个wxwxo 2 o2o 16 o 16家系的导入亲本供体片段最长为113.35 cM，最短为11.75 cM。这10个家系的BC 2F 4和BC 2F 3种子的赖氨酸含量差异不显著。这使我们能够提高糯玉米的赖氨酸含量，并生产出具有优良营养特性的种子，适用于人类消费、动物饲料和食品加工。这对优质玉米的选育和改善人、畜、禽的营养具有重要意义。
The low lysine content of waxy maize cannot meet the nutritional requirements of humans, livestock, or poultry. In the present study, the high-lysine genes o2 and o16 were backcrossed into wx lines using the maize high-lysine inbreds TAIXI19 (o2o2) and QCL3021 (o16o16) as donors and the waxy maize inbred line QCL5019 (wxwx) as a receptor. In the triple-cross F1, backcross, and inbred generations, the SSR markers phi027 and phi112 within the wx and o2 genes and the SSR marker umc1121 linked to the o16 gene were used for foreground selection. Background selection of the whole-genome SSR markers was performed for the selected individuals. The grain lysine content was determined using the dye-binding lysine method. The waxiness of the grain was determined with the I2-KI staining and dual-wavelength spectrophotometric analysis. The BC2F2 generation included 7 plants of genotype wxwxo2o2O16_, 19 plants of genotype wxwxo16o16O2_, and 3 plants of genotype wxwxo2o2o16o16. In these seeds, the average amylopectin content was 96.67%, 96.87%, and 96.62%, respectively, which is similar to that of QCL5019. The average lysine content was 0.555%, 0.380%, and 0.616%, respectively, representing increases of 75.1%, 19.9%, 94.3%, respectively, over QCL5019. The average genetic background recovery rate of the BC2F3 families was 95.3%, 94.3%, 94.2%, respectively. Among these 3 wxwxo2o2O16O16 families, 4 wxwxo2o2O16o16 families, and 3 wxwxo2o2o16o16 families, the longest imported parent donor fragment was 113.35 cM and the shortest fragment was 11.75 cM. No significant differences in lysine content were found between the BC2F4 seeds and the BC2F3 seeds in these 10 families. This allowed us to increase the lysine content of waxy corn and produce seeds with excellent nutritional characteristics suitable for human consumption, animal feed, and food processing. This may be of significance in the breeding of high-quality corn and in improvement of the nutrition of humans, livestock, and poultry.
登录
查看更多内容
DOI：
10.1016/0006-291x(62)90043-8
发表时间：
1962-01-01
影响因子：
3.1
作者：
NELSON, OE;RINES, HW
通讯作者：
RINES, HW
影响因子：
5.4
作者：
Ming, R;Brewbaker, JL;McMullen, MD
通讯作者：
McMullen, MD
DOI：
10.2307/2394586
发表时间：
1954-01-01
期刊：
Ann. Mo. Bot. Gdn.
影响因子：
--
作者：
Kuleshov, N. N.
通讯作者：
Kuleshov, N. N.
影响因子：
2.3
作者：
Schneider, KA;Brothers, ME;Kelly, JD
通讯作者：
Kelly, JD
DOI：
10.1007/bf00333960
发表时间：
1986-01-01
期刊：
MOLECULAR AND GENERAL GENETICS
影响因子：
--
作者：
KLOSGEN, RB;GIERL, A;SAEDLER, H
通讯作者：
SAEDLER, H