Genome-Wide Identification and Expression Analysis of Two-Component System Genes in Tomato.
Genome-Wide Identification and Expression Analysis of Two-Component System Genes in Tomato.
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DOI：
10.3390/ijms17081204
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发表时间：
2016-07-26
影响因子：
5.6
通讯作者：
Lu G
中科院分区：
文献类型：
--
作者：
He Y;Liu X;Ye L;Pan C;Chen L;Zou T;Lu G
由组氨酸激酶（HKs）、磷酸转移酶（HPs）和反应调节蛋白（RRs）组成的双组分系统（TCS）在调节植物生长、发育以及对生物和非生物胁迫的反应中起着关键作用。TCS基因已在多种作物中得到了广泛的鉴定和研究，但在番茄中的鉴定和研究却很少。本研究共从番茄基因组中鉴定出65个TCS基因，其中包括20个HK（L）、6个HP和39个RR。对TCS基因家族的分类、基因结构、保守结构域、染色体分布、系统发育关系、基因重复事件、亚细胞定位等进行了详细的预测和分析。番茄TCS家族成员的氨基酸序列除B型RRs外均高度保守。TCS家系的基因重复事件主要发生在RR家系。此外，RR的扩增归因于片段和串联重复。选择的绿色荧光蛋白（GFP）融合蛋白的亚细胞定位表现出多样的亚细胞靶向，从而证实了它们的预测的不同功能。大多数TCS家族成员表现出不同的器官或发育特异性表达模式。此外，大部分TCS基因是由非生物胁迫和外源激素诱导表达的。TCS元件的完整阐明将有助于全面分析TCS超家族的分子生物学和生理功能。
The two-component system (TCS), which comprises histidine kinases (HKs), phosphotransfers (HPs), and response regulator proteins (RRs), plays pivotal roles in regulating plant growth, development, and responses to biotic and abiotic stresses. TCS genes have been comprehensively identified and investigated in various crops but poorly characterized in tomato. In this work, a total of 65 TCS genes consisting of 20 HK(L)s, six HPs, and 39 RRs were identified from tomato genome. The classification, gene structures, conserved domains, chromosome distribution, phylogenetic relationship, gene duplication events, and subcellular localization of the TCS gene family were predicted and analyzed in detail. The amino acid sequences of tomato TCS family members, except those of type-B RRs, are highly conserved. The gene duplication events of the TCS family mainly occurred in the RR family. Furthermore, the expansion of RRs was attributed to both segment and tandem duplication. The subcellular localizations of the selected green fluorescent protein (GFP) fusion proteins exhibited a diverse subcellular targeting, thereby confirming their predicted divergent functionality. The majority of TCS family members showed distinct organ- or development-specific expression patterns. In addition, most of TCS genes were induced by abiotic stresses and exogenous phytohormones. The full elucidation of TCS elements will be helpful for comprehensive analysis of the molecular biology and physiological role of the TCS superfamily.
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影响因子：
1.6
作者：
Ishida, Kai;Yamashino, Takafumi;Mizuno, Takeshi
通讯作者：
Mizuno, Takeshi
DOI：
10.1093/dnares/dsu004
发表时间：
2014-08
期刊：
DNA research : an international journal for rapid publication of reports on genes and genomes
影响因子：
--
作者：
Liu Z;Zhang M;Kong L;Lv Y;Zou M;Lu G;Cao J;Yu X
通讯作者：
Yu X
影响因子：
2.9
作者：
Lam-Son Phan Tran;Shinozaki, Kazuo;Yamaguchi-Shinozaki, Kazuko
通讯作者：
Yamaguchi-Shinozaki, Kazuko
影响因子：
7.4
作者：
Pareek, Ashwani;Singh, Anupama;Singla-Pareek, Sneh L.
通讯作者：
Singla-Pareek, Sneh L.
DOI：
10.1093/dnares/dsp012
发表时间：
2009-08
期刊：
DNA research : an international journal for rapid publication of reports on genes and genomes
影响因子：
--
作者：
Ishida K;Niwa Y;Yamashino T;Mizuno T
通讯作者：
Mizuno T