Mechanism of SQQX Decoction's Protective Effect on SHR: A Serum Metabolomics-Based Analysis.
Mechanism of SQQX Decoction's Protective Effect on SHR: A Serum Metabolomics-Based Analysis.
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SQQX汤的机理对SHR的保护作用：基于血清代谢组学的分析。
DOI：
10.1155/2020/8856943
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发表时间：
2020
期刊：
影响因子：
--
通讯作者：
Huang L
中科院分区：
文献类型：
--
作者：
Wu J;Li L;Li L;Li Y;Xiao X;Qiao J;Sun R;Yang D;Yao R;Huang L
三七清轩汤是一种对高血压有较好疗效的药物制剂，由中国日中友好医院李黄教授根据30年的临床经验，在高血压病患者中被广泛接受为中药配方。既往研究表明，降血压作用可能是通过降低转化生长因子ββ等多种炎症因子水平，上调过氧化物酶体增殖物激活受体的表达来实现的。然而，从代谢学的角度研究SQQX对HTN的保护作用之前从未进行过。本研究旨在了解口服SQQX对自发性高血压大鼠(SHR)代谢谱的影响，寻找最佳剂量。干预组给予SQQX提取物低剂量(5.25 g/kg/d)、中剂量(10.5 g/kg/d)、高剂量(21 g/kg/d)灌胃90d。实验结束后处死大鼠，采集血清进行进一步检测。采用超高效液相色谱-串联质谱仪(UPLC/MS)分析血清代谢谱的变化。结果表明，SQQX能显著降低SHR的收缩压(SBP)，其中以高剂量组效果最好，与模型组比较，共11种代谢物均有明显变化。正交偏最小二乘判别分析(OPLS-DA)评分图显示，5组血清样本分为5类，高剂量组的代谢轨迹倾向于向对照组移动。甘鹅去氧胆酸、烟酰胺-N-氧化物和色氨酸甜菜碱可能是SQQX防治高血压的生物标志物，主要涉及胆固醇代谢、初级胆汁酸的生物合成、胆汁分泌、烟酸盐和烟酰胺代谢。综上所述，SQQX对SHR具有保护作用，其机制可能部分与恢复血清代谢紊乱有关。
SangQiQingXuan (SQQX) decoction is a pharmaceutical preparation exerting good therapeutic efficacy on high blood pressure (BP) and has widely been accepted in primarily hypertensive patients as a herbal formula prescribed by Professor Li Huang from China-Japan Friendship Hospital according to her 30-year clinical experience. A previous study showed that SQQX could reduce BP by decreasing levels of many inflammatory factors such as transforming growth factor beta (TGFβ) and elevating peroxisome proliferator activated receptor (PPAR) expression. However, a research focusing on SQQX's protection against HTN from a metabolomic perspective has never been done before. This study aimed to figure out the metabolic profiling variations due to oral administration of SQQX in spontaneous hypertensive rat (SHR) models and to find out the optimal dosage of SQQX. SHR in the intervention group orally received SQQX extract of three doses, namely, the low- (5.25 g/kg/d), middle- (10.5 g/kg/d), and high-dosage groups (21 g/kg/d) for 90 days. Rats were sacrificed at the end of the experiment, and their serum was collected for further examination. Serum metabolic profiling variations were analyzed using ultraperformance liquid chromatography coupled with tandem mass spectrometry (UPLC/MS). Results showed that dealing with SQQX remarkably decreased systolic blood pressure (SBP) of SHRs and the high-dosage group was with the best therapeutic effect where a total of 11 metabolites were markedly changed in contrast to the model group. Orthogonal partial least square discriminant analysis (OPLS-DA) score plot showed that the 5 groups of serum samples were divided into 5 categories, and the metabolic trajectory of the high-dosage SQQX group was inclined to move to the control group. Glycochenodeoxycholic acid, nicotinamide-N-oxide, and tryptophan betaine might be biomarkers that specifically marked the protective effects of SQQX against high BP mainly involving in cholesterol metabolism, primary bile acid biosynthesis, bile secretion, and nicotinate and nicotinamide metabolism. To conclude, SQQX has a protective effect on SHR, which may be partially correlated to restoration of perturbed metabolism in serum.
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影响因子：
6
作者：
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通讯作者：
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影响因子：
37.8
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影响因子：
2.6
作者：
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影响因子：
6.1
作者：
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通讯作者：
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基于LC-MS的血清代谢组学研究及钩藤对自发性高血压大鼠的降压作用
DOI：
10.1155/2018/9281946
发表时间：
2018
期刊：
Evidence-based complementary and alternative medicine : eCAM
影响因子：
--
作者：
Liu A;Chu YJ;Wang X;Yu R;Jiang H;Li Y;Zhou H;Gong LL;Yang WQ;Ju J
通讯作者：
Ju J