Aggregation-induced emission luminogen for in vivo three-photon fluorescence lifetime microscopic imaging
Aggregation-induced emission luminogen for in vivo three-photon fluorescence lifetime microscopic imaging
复制标题
用于体内三光子荧光寿命显微成像的聚集诱导发射发光体
DOI：
10.1142/s1793545819400054
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发表时间：
2019-09
影响因子：
2.5
通讯作者：
Jun Qian
中科院分区：
文献类型：
--
作者：
Huwei Ni;Zicong Xu;Dongyu Li;Ming Chen;Ben Zhong Tang;Jun Qian
与可见光相比，近红外（NIR）光在生物组织中具有更深的穿透性。三光子荧光显微镜（3 PFM）可以有效地利用近红外激发，以获得高对比度的图像在深部组织。然而，在传统的荧光强度成像模式下，微弱的三光子荧光信号可能不能很好地呈现。某些探针的荧光寿命对激发激光的强度不敏感。此外，荧光寿命成像显微镜（FLIM）可以利用时间相关单光子计数（TCSPC）技术检测微弱信号。因此，将3 PFM成像与FLIM结合在一起将是一种改进的策略。本文采用一种新型聚集诱导发射发光体（AIEgen）DCDPP-2 TPA作为荧光探针。具有深红色荧光发射的AIEgen的三光子吸收截面被证明是大的。合成了DCDPP-2 TPA纳米粒子，并测定了其在水中的三光子荧光寿命。此外，在体内的三光子荧光寿命显微成像的开颅手术小鼠进行了通过一个自制的光学系统。获得了不同垂直深度的高对比度脑血管图像，最大深度约为600 [公式：见正文]m。即使到达600米的深度，小至1.9米的毛细血管仍然可以分辨出来。毛细管的三光子荧光寿命与DCDPP-2 TPA纳米颗粒在水中的荧光寿命在某些代表性图像中雅阁。进一步组织生动的3D重建，以呈现丰富的终身信息。3 PFM和FLIM的结合策略有望在脑功能成像中得到进一步的应用。
Compared with visible light, near-infrared (NIR) light has deeper penetration in biological tissues. Three-photon fluorescence microscopy (3PFM) can effectively utilize the NIR excitation to obtain high-contrast images in the deep tissue. However, the weak three-photon fluorescence signals may be not well presented in the traditional fluorescence intensity imaging mode. Fluorescence lifetime of certain probes is insensitive to the intensity of the excitation laser. Moreover, fluorescence lifetime imaging microscopy (FLIM) can detect weak signals by utilizing time-correlated single photon counting (TCSPC) technique. Thus, it would be an improved strategy to combine the 3PFM imaging with the FLIM together. Herein, DCDPP-2TPA, a novel aggregation-induced emission luminogen (AIEgen), was adopted as the fluorescent probes. The three-photon absorption cross-section of the AIEgen, which has a deep-red fluorescence emission, was proved to be large. DCDPP-2TPA nanoparticles were synthesized, and the three-photon fluorescence lifetime of which was measured in water. Moreover, in vivo three-photon fluorescence lifetime microscopic imaging of a craniotomy mouse was conducted via a home-made optical system. High contrast cerebrovascular images of different vertical depths were obtained and the maximum depth was about 600 [Formula: see text]m. Even reaching the depth of 600 [Formula: see text]m, tiny capillary vessels as small as 1.9 [Formula: see text]m could still be distinguished. The three-photon fluorescence lifetimes of the capillaries in some representative images were in accord with that of DCDPP-2TPA nanoparticles in water. A vivid 3D reconstruction was further organized to present a wealth of lifetime information. In the future, the combination strategy of 3PFM and FLIM could be further applied in the brain functional imaging.
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影响因子：
13.3
作者：
Shang, Li;Azadfar, Naghmeh;Nienhaus, G. Ulrich
通讯作者：
Nienhaus, G. Ulrich
影响因子：
5
作者：
Esposito, Alessandro
通讯作者：
Esposito, Alessandro
影响因子：
10
作者：
Wang, Yalun;Han, Xiao;Qian, Jun
通讯作者：
Qian, Jun
无扫描多目标匹配多光子激发荧光显微镜
影响因子：
2.5
作者：
Junpeng Qiu;Lei Wang;Bruce Zhi Gao;Junle Qu;Yonghong Shao
通讯作者：
Yonghong Shao
影响因子：
5.8
作者：
M. Narenji;M. Talaee;H. Moghimi
通讯作者：
M. Narenji;M. Talaee;H. Moghimi