High-resolution melting analysis reveals genetic polymorphisms in microRNAs confer hepatocellular carcinoma risk in Chinese patients.
High-resolution melting analysis reveals genetic polymorphisms in microRNAs confer hepatocellular carcinoma risk in Chinese patients.
复制标题
高分辨率熔解分析揭示MicroRNA基因多态性与中国患者发生肝细胞癌的风险相关
DOI：
10.1186/1471-2407-14-643
复制
发表时间：
2014-08-31
期刊：
影响因子：
3.8
通讯作者：
Liu SM
中科院分区：
文献类型：
--
作者：
Qi JH;Wang J;Chen J;Shen F;Huang JT;Sen S;Zhou X;Liu SM
虽然microRNA （miRNA）基因的一些单核苷酸多态性与原发性肝细胞癌有关，但已发表的研究结果不一致且不确定。方法采用高分辨率熔融（HRM）分析，确定miR-146a C > G （rs2910164）、miR-196a2 C > T （rs11614913）、miR-301b A > G （rs384262）和miR-499 C > T （rs3746444）的snp在频率匹配的314例HCC患者和407例对照中，按年龄和性别是否存在差异。结果各组miR-196a2 C > T和miR-499 C > T基因型分布差异有统计学意义（P < 0.01），在不同显性遗传模型中，miR-196a2 C > T和miR-499 C > T基因型均增加肝癌发生风险（P < 0.01）；与携带一种或两种不利基因型的个体相比，携带两种不利基因型（CT + CC）的个体发生HCC的风险高出3.11倍(95%置信区间（CI）， 1.89-5.09；p = 7.18 × 10−6)。此外，两组患者miR-499 C > T等位基因频率差异有统计学意义，携带C等位基因的患者发生HCC的风险高于携带T等位基因的患者（优势比为1.53；95% CI为1.15 ~ 2.03;P = 0.003）。此外，我们发现miR-196a2 CC基因型HCC患者活化部分凝血活素时间（APTT）比TT基因型患者更长（P < 0.05）， miR-499 C等位基因HCC患者血清直接胆红素、球蛋白、γ-谷氨酰转肽酶、碱性磷酸酶水平较高，血清胆碱酯酶水平较低（P < 0.05）。结论我们的研究结果提示miR-196a2 C > T和miR-499 C > T的snp与HCC风险有关，并影响HCC患者的临床实验室特征。
BackgroundAlthough several single-nucleotide polymorphisms in microRNA (miRNA) genes have been associated with primary hepatocellular carcinoma, published findings regarding this relationship are inconsistent and inconclusive.MethodsThe high-resolution melting (HRM) analysis was used to determine whether the occurrence of the SNPs of miR-146a C > G (rs2910164), miR-196a2 C > T (rs11614913), miR-301b A > G (rs384262), and miR-499 C > T (rs3746444) differs in frequency-matched 314 HCC patients and 407 controls by age and sex.ResultsThe groups’ genotype distributions of miR-196a2 C > T and miR-499 C > T differed significantly (P < 0.01), both of them increased the risk of HCC in different dominant genetic models (P < 0.01); compared with individuals carrying one or neither of the unfavorable genotypes, individuals carrying both unfavorable genotypes (CT + CC) had a 3.11-fold higher HCC risk (95% confidence interval (CI), 1.89–5.09; P = 7.18 × 10−6). Moreover, the allele frequency of miR-499 C > T was significantly different between the two groups, and the HCC risk of carriers of the C allele was higher than that of carriers of the T allele (odds ratio, 1.53; 95% CI, 1.15-2.03; P = 0.003). Further, we found that the activated partial thromboplastin time (APTT) in HCC patients with miR-196a2 CC genotype was longer than patients with TT genotypes (P < 0.05), and HCC patients with miR-499 C allele had higher serum levels of direct bilirubin, globulin, γ-glutamyltranspeptidase, alkaline phosphatase, and lower serum cholinesterase (P < 0.05).ConclusionsOur findings suggest that the SNPs in miR-196a2 C > T and miR-499 C > T confer HCC risk and that affect the clinical laboratory characteristics of HCC patients.
登录
查看更多内容
影响因子：
29.4
作者：
El-Serag HB
通讯作者：
El-Serag HB
影响因子：
3.7
作者：
Hu M;Zhao L;Hu S;Yang J
通讯作者：
Yang J
DOI：
10.1073/pnas.0802682105
发表时间：
2008-05-20
影响因子：
11.1
作者：
Jazdzewski, Krystian;Murray, Elizabeth L.;de la Chapelle, Albert
通讯作者：
de la Chapelle, Albert
影响因子：
5
作者：
Lin, Pei-Chin;Liu, Ta-Chih;Chang, Jan-Gowth
通讯作者：
Chang, Jan-Gowth
pre-miRNA 的两种多态性与乳腺癌风险之间的关联：荟萃分析
影响因子：
3.8
作者：
Gao, Lin-Bo;Bai, Peng;Zhang, Lin
通讯作者：
Zhang, Lin