Immunogenicity of live phoP gene deletion strain of Riemerella anatipestifer serotype 1.
Immunogenicity of live phoP gene deletion strain of Riemerella anatipestifer serotype 1.
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1型鸭疫里默氏菌phoP基因缺失活株的免疫原性
DOI：
10.1016/j.psj.2022.102294
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发表时间：
2023-01
期刊：
影响因子：
4.4
通讯作者：
Li, Zili
中科院分区：
文献类型：
--
作者：
Li, Jian;Zhang, Yanhao;Wang, Ying;Zhang, Yang;Shi, Baolan;Gan, Luoxin;Yu, Shuang;Jia, Xiangchao;Yang, Kang;Li, Zili
鸭传染性浆膜炎是由鸭疫里默氏菌（R. anatipestier）引起的一种急性传染病，可导致鸭的周围肝炎、心包炎、脑膜炎和气囊炎，给全球鸭业造成严重的经济损失。phoP/phoR是一种新的双组分信号转导系统，首次在革兰氏阴性菌中报道，phoP作为全局调节剂和毒力因子。本研究采用同源重组技术敲除鸭疫鼠YM株phoP基因，代之以大观霉素耐药基因（Spec）。与野生型疫鼠疫鼠YM株相比，YMΔphoP株的毒力降低了约47000倍。以106 ~ 107 CFU （0.2 mL /只）的剂量皮下接种活禽疫鼠YMΔphoP株免疫鸭，14 d后再攻毒野生型禽疫鼠YM株。免疫组的保护率为100%。免疫组和阴性对照组鸭的生长特性正常，研究表明鸭疫R. YMΔphoP菌株具有适当的免疫原性和保护作用。因此，本研究结果提示，新型鸭疫R. YMΔphoP菌株可能为开发鸭传染性浆膜炎基因缺失激活疫苗提供候选株。
Duck infectious serositis is an acute and infectious disease caused by Riemerella anatipestifer (R. anatipestifer) that leads to perihepatitis, pericarditis, meningitis, and airbag inflammation in ducks, which causes serious economic losses to the global duck industry. The phoP/phoR is a novel 2-component signal transduction system first reported in gram-negative bacteria, of which phoP acts as a global regulator and virulence factor. In this study, the phoP gene from the R. anatipestifer YM strain was knocked out using homologous recombination technology and replaced with the spectinomycin resistance gene (Spec). The virulence of the R. anatipestifer YMΔphoP strain was reduced by approximately 47,000 times compared to that of the wild-type R. anatipestifer YM strain. Ducks were immunized with live R. anatipestifer YMΔphoP strain by subcutaneous inoculation at a dose of 106 to 107 CFU (0.2 mL per duck) and challenged with the wild-type R. anatipestifer YM strain 14 days later. The protection rate in the immunized group was 100%. The growth characteristics of ducks in the immunized and negative control groups were normal, and the research demonstrated R. anatipestifer YMΔphoP strain have suitable immunogenicity and protective effects. Thus, the study findings suggest that the novel R. anatipestifer YMΔphoP strain may provide a candidate for the development of a gene deletion activated vaccine against duck infectious serositis.
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影响因子：
4.4
作者：
Dou Y;Yu G;Wang X;Wang S;Li T;Tian M;Qi J;Ding C;Yu S
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3.1
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作者：
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通讯作者：
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3.3
作者：
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