LncRNA FGD5-AS1 functions as an oncogene to upregulate GTPBP4 expression by sponging miR-873-5p in hepatocellular carcinoma.
LncRNA FGD5-AS1 functions as an oncogene to upregulate GTPBP4 expression by sponging miR-873-5p in hepatocellular carcinoma.
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LncRNA FGD5-AS1 作为癌基因通过海绵 miR-873-5p 在肝细胞癌中上调 GTPBP4 表达
DOI：
10.4081/ejh.2021.3300
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发表时间：
2021-11-16
期刊：
影响因子：
--
通讯作者：
Chen X
中科院分区：
文献类型：
--
作者：
Zhang N;Shen H;Huang S;Wang F;Liu H;Xie F;Jiang L;Chen X
长链非编码FGD 5-AS 1（LncFGD 5-AS 1）是一种新的致癌基因，通过海绵状microRNAs（miRNAs）参与调节肿瘤的进展。然而，FGD 5-AS 1在肝细胞癌（HCC）中的表达模式和生物学作用仍不清楚。采用RT-qPCR检测FGD 5-AS 1在肿瘤组织和细胞系中的表达水平。通过CCK-8、EdU、流式细胞术、创伤愈合和transwell小室分析来研究FGD 5-AS 1在HCC细胞增殖、凋亡、迁移和侵袭中的作用。进行双荧光素酶报告基因和RNA下拉测定以鉴定FGD 5-AS 1、miR-873- 5 p和GTP结合蛋白4（GTPBP 4）之间的调控相互作用。我们发现FGD 5-AS 1在HCC组织和细胞系中表达上调。此外，FGD 5-AS 1的敲低抑制肝癌细胞的增殖、迁移和侵袭，并诱导肝癌细胞凋亡。进一步的研究表明，FGD 5-AS 1可以作为竞争性RNA在HCC细胞中海绵状地吸收miR-873- 5 p。GTPBP 4是miR-873- 5 p的直接下游靶点，FGD 5-AS 1通过与miR-873- 5 p竞争性结合介导GTPBP 4的作用。综上所述，本研究证明了FGD 5-AS 1在HCC进展中的调节作用，并将miR-873- 5 p/GTPBP 4轴确定为直接下游途径。它为HCC患者提供了一种有前途的新治疗策略。
The long non-coding FGD5-AS1 (LncFGD5-AS1) has been reported to be a novel carcinogenic gene and participant in regulating tumor progression by sponging microRNAs (miRNAs). However, the pattern of expression and the biological role of FGD5-AS1 in hepatocellular carcinoma (HCC) remains largely unknown. The expression level of FGD5-AS1 in tumor tissues and cell lines was measured by RT-qPCR. CCK-8, EdU, flow cytometry, wound healing and transwell chamber assays were performed to investigate the role of FGD5-AS1 in cell proliferation, apoptosis, migration, and invasion in HCC. Dual luciferase reporter, and RNA pull-down assays were performed to identify the regulatory interactions among FGD5-AS1, miR-873-5p and GTP-binding protein 4 (GTPBP4). We found that the expression of FGD5-AS1 was upregulated in HCC tissues and cell lines. Moreover, the knockdown of FGD5-AS1 suppressed cell proliferation, migration and invasion, and induced apoptosis in HCC cells. Further studies demonstrated that FGD5-AS1 could function as a competitive RNA by sponging miR-873-5p in HCC cells. Moreover, GTPBP4 was identified as direct downstream target of miR-873-5p in HCC cells and FGD5-AS1mediated the effects of GTPBP4 by competitively binding with miR-873-5p. Taken together, this study demonstrated the regulatory role of FGD5-AS1 in the progression of HCC and identified the miR-873-5p/GTPBP4 axis as the direct downstream pathway. It represents a promising novel therapeutic strategy for HCC patients.
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用于肝细胞癌诊断、预后和治疗的生物标志物 MicroRNA：功能调查和比较
影响因子：
4.6
作者：
Shen S;Lin Y;Yuan X;Shen L;Chen J;Chen L;Qin L;Shen B
通讯作者：
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影响因子：
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作者：
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Zhao H
DOI：
10.1080/21691401.2018.1544143
发表时间：
2019-01-01
影响因子：
5.8
作者：
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通讯作者：
Song, Hang
DOI：
10.1007/978-981-10-5203-3_7
发表时间：
2017-01-01
期刊：
LONG NON CODING RNA BIOLOGY
影响因子：
--
作者：
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通讯作者：
Felley-Bosco, Emanuela
影响因子：
8
作者：
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通讯作者：
Edgington, T. S.