Identification of CDF family members in apple and their expression in response to sucrose during floral induction
Identification of CDF family members in apple and their expression in response to sucrose during floral induction
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苹果 CDF 家族成员的鉴定及其在花诱导过程中对蔗糖反应的表达
DOI：
10.1080/17429145.2020.1760951
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发表时间：
2020-01
影响因子：
3.2
通讯作者：
Xing Libo
中科院分区：
文献类型：
--
作者：
Qi Siyan;Ma Wenchun;An Na;Gao Xiuhua;Du Lisha;Zhang Dong;Ma Juanjuan;Zhao Caiping;Han Mingyu;Xing Libo
植物特异性CDF基因编码的一类转录因子在植物开花，特别是光周期调控开花中起着至关重要的作用。但对MdCDFs在苹果花诱导过程中的潜在功能了解甚少。在本研究中，系统地鉴定和命名了11个假定的MdCDFs。生物信息学分析揭示了MdCDFs在功能和结构上的相似性。对苹果和拟南芥的CDF序列进行串联和同源性分析。RT-qPCR结果显示，MdCDFs在苹果不同组织中表现出不同的表达模式，其中大部分在花和叶中表达量较高。进一步研究了MdCDFs在苹果不同开花品种和糖处理下的表达谱。结果表明，MdCDFs对花诱导有响应。同时，糖处理后芽体中所有MdCDFs的表达在诱导花的不同阶段均受到抑制。开花相关基因和光周期通路相关基因的表达存在差异，这可能表明大多数MdCDFs可能具有光响应开花抑制剂的功能，并可能被糖介导的花诱导抑制。总的来说，对MdCDFs的综合分析提供了它们在花诱导过程中表达的有价值的信息，这将为进一步的研究奠定基础。
ABSTRACT Plant-specific CDF genes encode a class of transcription factors which play crucial roles in plant flowering, especially in photoperiod-regulated flowering. But little is known about the potential functions of MdCDFs during flower induction in apple. In this study, 11 putative MdCDFs were identified and named systematically. Bioinformatic analysis revealed a functional and structural similarity among the MdCDFs. Analysis of tandem and synteny revealed several paired CDF homologs between apple and Arabidopsis. RT-qPCR revealed that MdCDFs exhibited distinct expression patterns in different apple tissues and most of them displayed high expression levels in flowers and leaves. Further expression profiles of MdCDFs were investigated in different-flowering varieties of apple and in response to sugar treatment. The results indicated that MdCDFs were responsive to floral induction. Meanwhile, the expressions of all MdCDFs were inhibited in buds during different floral induction stages after sugar treatment. The expressions of flowering-related and photoperiod pathway-related genes showed differences and may indicate that most of the MdCDFs may function as a light response flowering inhibitor and could be repressed by the sugar-mediated floral induction. Collectively, the comprehensive analysis of MdCDFs provides valuable information on their expression during floral induction that will serve as a foundation for further studies.
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影响因子：
8.7
作者：
da Silva, Danielle Costenaro;Falavigna, Vitor da Silveira;Fasoli, Marianna;Buffon, Vanessa;Porto, Diogo Denardi;Pappas, Georgios Joannis, Jr.;Pezzotti, Mario;Pasquali, Giancarlo;Revers, Luis Fernando
通讯作者：
Revers, Luis Fernando
DOI：
10.1016/j.plaphy.2014.03.011
发表时间：
2014-06
期刊：
Plant physiology and biochemistry : PPB
影响因子：
--
作者：
Yanxiao Tan;Suncai Wang;D. Liang;Mingjun Li;F. Ma
通讯作者：
Yanxiao Tan;Suncai Wang;D. Liang;Mingjun Li;F. Ma
影响因子：
1.9
作者：
H. Caspari;A. Lang;P. Alspach
通讯作者：
H. Caspari;A. Lang;P. Alspach
影响因子：
6.9
作者：
Corrales, Alba-Rocio;Nebauer, Sergio G.;Medina, Joaquin
通讯作者：
Medina, Joaquin
影响因子：
64.8
作者：
Kloosterman, Bjorn;Abelenda, Jose A.;Bachem, Christian W. B.
通讯作者：
Bachem, Christian W. B.