Divergent innervation of the olfactory bulb by distinct raphe nuclei.
Divergent innervation of the olfactory bulb by distinct raphe nuclei.
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DOI：
10.1002/cne.23713
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发表时间：
2015-04-01
影响因子：
2.5
通讯作者：
Fukunaga, Izumi
中科院分区：
文献类型：
--
作者：
Steinfeld, Raphael;Herb, Jan T.;Sprengel, Rolf;Schaefer, Andreas T.;Fukunaga, Izumi
中缝核在脑内广泛提供多巴胺能神经支配，被认为介导了多种神经调节作用。哺乳动物嗅球（OB）是嗅觉神经能纤维的重要受体，特别是在肾小球层（GL）中，它们被认为是来自嗅觉神经的传入信号的门控。中缝背核（DRN）和中缝正中核（MRN）密集地支配OB。大多数这种预测被认为在GL中终止，但这还没有得到明确的测试。我们试图研究这一点，使用重组腺相关病毒（rAAV）介导的表达绿色荧光蛋白（GFP）-突触体素专门针对神经元的DRN或MRN。DRN注射后，标记纤维主要位于颗粒细胞层（GCL），而不是GL。相反，在GL中观察到密集的标记与MRN注射，这表明GL神经支配的来源是MRN，而不是DRN，如先前所认为的。因此，两个中缝核在OB内给予双重神经支配，具有不同的神经支配模式。神经学比较杂志523：805-813，2015.© 2015 Wiley Periodicals，Inc.
The raphe nuclei provide serotonergic innervation widely in the brain, thought to mediate a variety of neuromodulatory effects. The mammalian olfactory bulb (OB) is a prominent recipient of serotonergic fibers, particularly in the glomerular layer (GL), where they are thought to gate incoming signals from the olfactory nerve. The dorsal raphe nucleus (DRN) and the median raphe nucleus (MRN) are known to densely innervate the OB. The majority of such projections are thought to terminate in the GL, but this has not been explicitly tested. We sought to investigate this using recombinant adeno-associated viruses (rAAV)-mediated expression of green fluorescent protein (GFP)-synaptophysin targeted specifically to neurons of the DRN or the MRN. With DRN injections, labeled fibers were found mostly in the granule cell layer (GCL), not the GL. Conversely, dense labeling in the GL was observed with MRN injections, suggesting that the source of GL innervation is the MRN, not the DRN, as previously thought. The two raphe nuclei thus give dual innervation within the OB, with distinct innervation patterns. J. Comp. Neurol. 523:805–813, 2015. © 2015 Wiley Periodicals, Inc.
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