Induction of Rhesus Keratinocytes into Functional Ameloblasts by Mouse Embryonic Dental Mesenchyme
Induction of Rhesus Keratinocytes into Functional Ameloblasts by Mouse Embryonic Dental Mesenchyme
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小鼠胚胎牙齿间充质诱导恒河猴角质形成细胞形成功能性成釉细胞
DOI：
10.1007/s13770-017-0098-2
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发表时间：
2017-12
期刊：
影响因子：
--
通讯作者：
ing
中科院分区：
文献类型：
--
作者：
Ruan ningsheng;Lin chensheng;Dong xiuqing;Hu xuefeng;Zhang y;ing
近年来，以干细胞为基础的牙齿组织工程在小鼠、大鼠、狗、猪等动物模型上取得了快速进展。此外，已经分离出多种牙齿来源的出生后间充质干细胞，并显示其能够分化为成牙本质细胞并生成牙本质。同时，人类角质形成细胞干/祖细胞，牙龈上皮细胞，甚至iPSC衍生的上皮细胞已被证明能够分化为功能性成釉细胞。在非人灵长类动物中进行转化医学研究是干细胞组织工程治疗临床应用不可替代的步骤。在本研究中，我们首先检查上皮干细胞标记在恒河猴皮肤使用免疫染色。然后从恒河猴表皮中分离角质形成细胞干细胞，体外培养，并通过上皮干细胞标记进行鉴定。这些培养的角质形成细胞，这是重组E13.5小鼠牙间充质细胞，具有牙源性的潜力，在外源性FGF 8的存在下，上皮片诱导分化成釉分泌成釉细胞。我们的研究结果表明，在适当的牙源性信号的存在下，恒河猴角质形成细胞可以被诱导获得牙源性能力，并能够参与牙源性，表明恒河猴角质形成细胞是一个理想的上皮细胞来源，进一步转化医学研究的牙齿组织工程在非人灵长类动物。
Fast progresses in stem cell-based tooth tissue engineering have been achieved in recent years in several animal models including the mouse, rat, dog, and pig. Moreover, various postnatal mesenchymal stem cells of dental origin have been isolated and shown capable of differentiating into odontoblasts and generating dentin. Meanwhile, human keratinocyte stem/progenitor cells, gingival epithelial cells, and even iPSC-derived epithelium have been demonstrated to be able to differentiate into functional ameloblasts. Translational medicine studies in the nonhuman primate are irreplaceable steps towards clinical application of stem cell-based tissue engineering therapy. In the present study, we first examined the epithelial stem cell markers in the rhesus skin using immunostaining. Keratinocyte stem cells were then isolated from rhesus epidermis, culturedin vitro, and characterized by epithelial stem cell markers. Epithelial sheets of these cultured keratinocytes, which were recombined with E13.5 mouse dental mesenchyme that possesses odontogenic potential in the presence of exogenous FGF8, were induced to differentiate into enamel-secreting ameloblasts. Our results demonstrate that in the presence of appropriate odontogenic signals, rhesus keratinocytes can be induced to gain odontogenic competence and are capable of participating in odontogenesis, indicating that rhesus keratinocytes are an ideal epithelial cell source for further translational medicine study of tooth tissue engineering in nonhuman primates.
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影响因子：
3.7
作者：
Oshima M;Mizuno M;Imamura A;Ogawa M;Yasukawa M;Yamazaki H;Morita R;Ikeda E;Nakao K;Takano-Yamamoto T;Kasugai S;Saito M;Tsuji T
通讯作者：
Tsuji T
利用免整合的人尿诱导多能干细胞生成齿状结构
DOI：
10.1186/2045-9769-2-6
发表时间：
2013
期刊：
Cell regeneration (London, England)
影响因子：
--
作者：
Cai J;Zhang Y;Liu P;Chen S;Wu X;Sun Y;Li A;Huang K;Luo R;Wang L;Liu Y;Zhou T;Wei S;Pan G;Pei D
通讯作者：
Pei D
影响因子：
6.5
作者：
Waseem, A;Dogan, B;Leigh, IM
通讯作者：
Leigh, IM
影响因子：
4.8
作者：
Weibo Zhang;H. Abukawa;M. Troulis;L. Kaban;J. Vacanti;P. Yelick
通讯作者：
Weibo Zhang;H. Abukawa;M. Troulis;L. Kaban;J. Vacanti;P. Yelick
影响因子：
7.6
作者：
Huang GT;Gronthos S;Shi S
通讯作者：
Shi S