Microarchitectural and mechanical characterization of the sickle bone.
Microarchitectural and mechanical characterization of the sickle bone.
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DOI：
10.1016/j.jmbbm.2015.04.019
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发表时间：
2015-08
影响因子：
3.9
通讯作者：
Barabino G
中科院分区：
文献类型：
--
作者：
Green M;Akinsami I;Lin A;Banton S;Ghosh S;Chen B;Platt M;Osunkwo I;Ofori-Acquah S;Guldberg R;Barabino G
镰状细胞病患者经常经历急性和慢性骨痛，这是由于组织血管内闭塞事件导致缺血、坏死和器官变性。从宏观上看，镰状骨在临床x线片上表现为矿物质密度降低、骨髓腔变宽、皮质骨变薄，这是由于该病伴红系增生升高所致。然而，镰状骨的微观结构及其在机械功能中的作用在很大程度上是未知的。本研究利用micro-CT和生物力学测试来确定转基因镰状雄鼠和具有镰状性状的窝鼠以及野生型对照10周龄和21周龄股骨的骨形态、组织矿物质密度、骨小梁和皮质微结构之间的关系。虽然骨组织矿物质密度在两个年龄的基因型之间没有变化，但观察到骨微观结构的变化。在10周时，健康小鼠和性状小鼠在皮质骨和骨小梁内表现出相似的形态，而镰状小鼠表现出高度连接的骨小梁。在老年股骨中，镰状和性状标本显示的小梁明显较少，并且根据结构模型指数，剩余的小梁具有更恶化的几何形状。镰状股骨皮质区域变薄有助于在10周和21周大时表现出明显低于对照组的弹性模量的灵活性。野生型和性状型股骨通常表现出相似的力学性能；然而，性状股骨在21周时的模数明显高于镰刀型和野生型对照。总的来说，这些数据表明镰状细胞病引起的微血管进行性损伤导致骨组织微结构和力学的有害结构变化。
Individuals with sickle cell disease often experience acute and chronic bone pain due to occlusive events within the tissue vasculature that result in ischemia, necrosis, and organ degeneration. Macroscopically, sickle bone is identified in clinical radiographs by its reduced mineral density, widening of the marrow cavity, and thinning of the cortical bone due to the elevated erythroid hyperplasia accompanying the disease. However, the microstructural architecture of sickle bone and its role in mechanical functionality is largely unknown. This study utilized micro-CT and biomechanical testing to determine the relationship between the bone morphology, tissue mineral density, and trabecular and cortical microarchitecture of 10-and 21-week-old femurs from transgenic sickle male mice and littermates with sickle trait, as well as a wild-type control. While bone tissue mineral density did not vary among the genotypes at either age, variation in bone microstructure were observed. At 10 weeks, healthy and trait mice exhibited similar morphology within the cortical and trabecular bone, while sickle mice exhibited highly connected trabeculae. Within older femurs, sickle and trait specimens displayed significantly fewer trabeculae, and the remaining trabeculae had a more deteriorated geometry based on the structure model index. Thinning of the cortical region in sickle femurs contributed to the displayed flexibility with a significantly lower elastic modulus than the controls at both 10- and 21-weeks old. Wild-type and trait femurs generally demonstrated similar mechanical properties; however, trait femurs had a significantly higher modulus than sickle and wild-type control at 21-weeks. Overall, these data indicate that the progressive damage to the microvasculature caused by sickle cell disease, results in deleterious structural changes in the bone tissue's microarchitecture and mechanics.
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影响因子：
2.4
作者：
Aguilar, C;Vichinsky, E;Neumayr, L
通讯作者：
Neumayr, L
影响因子：
3.2
作者：
Fung, Ellen B.;Kawchak, Deborah A.;Stallings, Virginia A.
通讯作者：
Stallings, Virginia A.
影响因子：
2.4
作者：
GIBSON, LJ
通讯作者：
GIBSON, LJ
影响因子：
4.1
作者：
Arlet, Jean-Benoit;Courbebaisse, Marie;Ribeil, Jean-Antoine
通讯作者：
Ribeil, Jean-Antoine
DOI：
10.1080/10739680802279394
发表时间：
2009-01
期刊：
Microcirculation (New York, N.Y. : 1994)
影响因子：
--
作者：
Kaul DK;Finnegan E;Barabino GA
通讯作者：
Barabino GA