Sensory Neurons Arouse C. elegans Locomotion via Both Glutamate and Neuropeptide Release.
Sensory Neurons Arouse C. elegans Locomotion via Both Glutamate and Neuropeptide Release.
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DOI：
10.1371/journal.pgen.1005359
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发表时间：
2015-07
期刊：
影响因子：
4.5
通讯作者：
Kaplan JM
中科院分区：
文献类型：
--
作者：
Choi S;Taylor KP;Chatzigeorgiou M;Hu Z;Schafer WR;Kaplan JM
C.秀丽线虫在幼虫蜕皮期间（称为昏睡期）和作为成虫经历行为静止期。很少有人知道的电路机制，建立这些静态。在缺乏神经肽受体NPR-1的突变体中，Lethargus和成体运动静止显著减少。在这里，我们表明，引起的运动npr-1突变体的结果夸大的活动，在多个类别的感觉神经元，包括伤害性（ASH），触摸敏感（ALM和PLM），和拉伸感觉（DVA）神经元。这些感觉神经元通过神经肽和谷氨酸的释放来加速运动。这些感觉神经元对唤醒的相对贡献在幼虫蜕皮和成虫之间不同。我们的研究结果表明，一个广泛的感觉神经元网络决定了唤醒和静止行为状态之间的转换。动物会根据环境、发育和昼夜节律的线索，在行为唤醒和静止期之间切换。我们对产生这些行为状态的电路机制知之甚少。在幼虫蜕皮期间，C.秀丽线虫表现出一种类似睡眠的状态（称为昏睡），其特征在于没有进食和深度运动静止。我们以前表明，缺乏神经肽受体NPR-1的突变体表现出增加唤醒幼虫蜕皮期间，这是部分介导的唤醒肽（PDF-1）的分泌增加。在这里，我们比较电路调节唤醒幼虫蜕皮和成人。我们发现，一个广泛的感觉神经元网络引起运动，但每个神经元的影响不同昏睡和成人之间。我们认为，这种广泛的感觉网络允许C。在广泛的发育和生理环境中适应其行为。
C. elegans undergoes periods of behavioral quiescence during larval molts (termed lethargus) and as adults. Little is known about the circuit mechanisms that establish these quiescent states. Lethargus and adult locomotion quiescence is dramatically reduced in mutants lacking the neuropeptide receptor NPR-1. Here, we show that the aroused locomotion of npr-1 mutants results from the exaggerated activity in multiple classes of sensory neurons, including nociceptive (ASH), touch sensitive (ALM and PLM), and stretch sensing (DVA) neurons. These sensory neurons accelerate locomotion via both neuropeptide and glutamate release. The relative contribution of these sensory neurons to arousal differs between larval molts and adults. Our results suggest that a broad network of sensory neurons dictates transitions between aroused and quiescent behavioral states. Animals switch between periods of behavioral arousal and quiescence in response to environmental, developmental, and circadian cues. Little is known about the circuit mechanisms that produce these behavioral states. During larval molts, C. elegans exhibits a sleep-like state (termed lethargus) that is characterized by the absence of feeding and profound locomotion quiescence. We previously showed that mutants lacking the neuropeptide receptor NPR-1 exhibit increased arousal during larval molts, which is in part mediated by increased secretion of an arousal peptide (PDF-1). Here, we compare the circuits regulating arousal in larval molts and adults. We show that a broad network of sensory neurons arouses locomotion but that the impact of each neuron differs between lethargus and adults. We propose that this broad sensory network allows C. elegans to adapt its behavior across a broad range of developmental and physiological circumstances.
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影响因子：
16.2
作者：
Hu Z;Pym EC;Babu K;Vashlishan Murray AB;Kaplan JM
通讯作者：
Kaplan JM
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通讯作者：
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