Temperature-sensitive albino gene TCD5, encoding a monooxygenase, affects chloroplast development at low temperatures.
Temperature-sensitive albino gene TCD5, encoding a monooxygenase, affects chloroplast development at low temperatures.
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编码单加氧酶的温度敏感白化基因 TCD5 会影响低温下叶绿体的发育。
DOI：
10.1093/jxb/erw287
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发表时间：
2016-09
影响因子：
6.9
通讯作者：
Teng S
中科院分区：
文献类型：
--
作者：
Wang Y;Zhang J;Shi X;Peng Y;Li P;Lin D;Dong Y;Teng S
一个新的温度敏感白化病基因TCD 5编码一个单加氧酶，在低温下影响水稻P4期叶绿体的发育。叶绿体是光合作用所必需的，在植物发育中起着关键作用。在这项研究中，我们对温度敏感的叶绿素缺乏型水稻突变体tcd 5进行了表征，该突变体在低温（20 °C）下形成白化病叶，在高温（32 °C）下形成正常的绿色叶。在20 °C下，tcd 5植物中叶绿体和致病体的发育受损，并且白化病表型的温度敏感期是叶发育的P4阶段。在20 °C下，tcd 5植物中类囊体膜的发育在P4中期被阻止。我们进行了TCD 5的定位克隆，然后进行了互补和敲低实验，结果表明来自LOC_Os05g34040基因的转录本LOC_Os05g34040.1对应于tcd 5表型。TCD 5编码一个保守的质体靶向单加氧酶家族蛋白，该蛋白以前没有报道与植物中的温度敏感性白化病表型相关。TCD 5在幼叶和未成熟穗中大量表达，低温增加了这种表达。在tcd 5突变体中，一些与质体转录/翻译和光合作用有关的基因的转录发生了变化。尽管TCD 5正向突变体在水稻和拟南芥中的表型和温度依赖性不同，但OsTCD 5可以拯救拟南芥突变体的表型，表明TCD 5功能在单子叶植物和双子叶植物中是保守的。
A new temperature-sensitive albino gene, TCD5, encoding a monooxygenase, affects chloroplast development at P4 stage under low temperature in rice. Chloroplasts are essential for photosynthesis and play critical roles in plant development. In this study, we characterized the temperature-sensitive chlorophyll-deficient rice mutant tcd5, which develops albino leaves at low temperatures (20 °C) and normal green leaves at high temperatures (32 °C). The development of chloroplasts and etioplasts is impaired in tcd5 plants at 20 °C, and the temperature-sensitive period for the albino phenotype is the P4 stage of leaf development. The development of thylakoid membranes is arrested at the mid-P4 stage in tcd5 plants at 20 °C. We performed positional cloning of TCD5 and then complementation and knock-down experiments, and the results showed that the transcript LOC_Os05g34040.1 from the LOC_Os05g34040 gene corresponded to the tcd5 phenotype. TCD5 encodes a conserved plastid-targeted monooxygenase family protein which has not been previously reported associated with a temperature-sensitive albino phenotype in plants. TCD5 is abundantly expressed in young leaves and immature spikes, and low temperatures increased this expression. The transcription of some genes involved in plastid transcription/translation and photosynthesis varied in the tcd5 mutant. Although the phenotype and temperature dependence of the TCD5 orthologous mutant phenotype were different in rice and Arabidopsis, OsTCD5 could rescue the phenotype of the Arabidopsis mutant, suggesting that TCD5 function is conserved between monocots and dicots.
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影响因子：
1.9
作者：
MONJE, OA;BUGBEE, B
通讯作者：
BUGBEE, B
影响因子：
1.6
作者：
Kusumi, Kensuke;Chono, Yoko;Iba, Koh
通讯作者：
Iba, Koh
影响因子：
11.4
作者：
Hajdukiewicz, PTJ;Allison, LA;Maliga, P
通讯作者：
Maliga, P
DOI：
10.12703/p6-40
发表时间：
2014
期刊：
F1000prime reports
影响因子：
--
作者：
Jensen PE;Leister D
通讯作者：
Leister D
影响因子：
2.4
作者：
Dong, YJ;Dong, WQ;Jin, QS
通讯作者：
Jin, QS