Allelic variation partially regulates galactose-dependent hydrogen peroxide release from circulating hemocytes of the snail Biomphalaria glabrata.
Allelic variation partially regulates galactose-dependent hydrogen peroxide release from circulating hemocytes of the snail Biomphalaria glabrata.
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DOI：
10.1016/j.fsi.2017.10.037
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发表时间：
2018-01
影响因子：
4.7
通讯作者：
Blouin MS
中科院分区：
文献类型：
--
作者：
Allan ERO;Blouin MS
淡水蜗牛是许多对人类和农业健康有害的寄生蠕虫的中间宿主。了解这些蜗牛的免疫反应对于找到阻止这些寄生虫传播的方法至关重要。等位基因变异在最近发现的基因组区域中的蜗牛，双脐螺光滑，影响他们的易感性，染色体。在这里，我们测试了该区域的基因，称为瓜德罗普抗性复合体（GRC），是否参与识别常见的病原体相关分子，这些分子已被证明是过氧化氢防御途径的刺激物。我们发现，从三个GRC基因型之一的个人提取的血细胞释放的过氧化氢比其他两种基因型，半乳糖刺激后。在用几种其他微生物相关的碳水化合物刺激后没有观察到这种差异，尽管这些配体共享相同的过氧化氢释放的推定途径。因此，我们得出结论，在GRC区域的等位基因变异可能会影响半乳糖的识别，而不是保守的过氧化氢途径的下游步骤。因此，这些结果与该区域产生的蛋白质参与病原体识别的假设一致。
Freshwater snails are the intermediate hosts for numerous parasitic worms that are detrimental to human and agricultural health. Understanding the immune responses of these snails could be vital for finding ways to block transmission of those parasites. Allelic variation in a recently discovered genomic region in the snail, Biomphalaria glabrata, influences their susceptibility to schistosomes. Here we tested whether genes in that region, termed the Guadeloupe Resistance Complex (GRC), are involved in recognition of common pathogen-associated molecules that have been shown to be stimulants of the hydrogen peroxide defense pathway. We show that hemocytes extracted from individuals with one of the three GRC genotypes released less hydrogen peroxide than the other two genotypes, after stimulation with galactose. This difference was not observed after stimulation with several other microbial-associated carbohydrates, despite those ligands sharing the same putative pathway for hydrogen peroxide release. Therefore, we conclude that allelic variation in the GRC region may influence the recognition of galactose, rather than the conserved downstream steps in the hydrogen peroxide pathway. These results thus are consistent with the hypothesis that proteins produced by this region are involved in pathogen recognition.
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影响因子：
1.3
作者：
Basso, W;Venturini, L;Dubey, JP
通讯作者：
Dubey, JP
影响因子：
1.3
作者：
Bender, RC;Broderick, EJ;Bayne, CJ
通讯作者：
Bayne, CJ
影响因子：
2.9
作者：
Jiang, Yiguo;Loker, Eric S.;Zhang, Si-Ming
通讯作者：
Zhang, Si-Ming
影响因子：
4
作者：
Humphries, JE;Yoshino, TP
通讯作者：
Yoshino, TP
影响因子：
2.9
作者：
Hahn, UK;Bender, RC;Bayne, CJ
通讯作者：
Bayne, CJ