Internalization of Staphylococcus aureus in lymphocytes induces oxidative stress and DNA fragmentation: possible ameliorative role of nanoconjugated vancomycin.
Internalization of Staphylococcus aureus in lymphocytes induces oxidative stress and DNA fragmentation: possible ameliorative role of nanoconjugated vancomycin.
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DOI：
10.1155/2011/942123
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发表时间：
2011
影响因子：
--
通讯作者：
Roy S
中科院分区：
文献类型：
--
作者：
Chakraborty SP;Kar Mahapatra S;Sahu SK;Das S;Tripathy S;Dash S;Pramanik P;Roy S
金黄色葡萄球菌是引起血液感染、皮肤和软组织感染以及肺炎的最常见的分离病原体。淋巴细胞是一种重要的免疫细胞。本论文的目的是测试纳米结合万古霉素对万古霉素敏感的金黄色葡萄球菌(VSSA)和耐万古霉素的金黄色葡萄球菌(VRSA)感染诱导的淋巴细胞氧化应激的改善作用。给瑞士小鼠腹腔注射5×106CFU/mLVSSA和VRSA5×106CFU/ 的细菌溶液，建立感染模型。给感染VSSA和VRSA的小鼠以有效剂量给予纳米结合万古霉素10天。给感染VSSA和VRSA的小鼠分别给予相同剂量的万古霉素，连续10天。正常小鼠按有效剂量给予万古霉素和纳米万古霉素，连续给药10天。研究结果表明，在体内，VSSA和VRSA感染显著增加了脂质过氧化、蛋白质氧化、氧化谷胱甘肽水平、亚硝酸盐生成、亚硝酸盐释放和DNA损伤，降低了还原型谷胱甘肽、抗氧化酶状态和谷胱甘肽依赖酶的水平，在纳米结合万古霉素处理组，这些水平明显升高或降低，接近正常水平。这些发现提示了纳米结合万古霉素在对抗VSSA和VRSA感染诱导的淋巴细胞氧化应激中的潜在用途和有益作用。
Staphylococcus aureus is the most frequently isolated pathogen causing bloodstream infections, skin and soft tissue infections and pneumonia. Lymphocyte is an important immune cell. The aim of the present paper was to test the ameliorative role of nanoconjugated vancomycin against Vancomycin-sensitive Staphylococcus aureus (VSSA) and vancomycin-resistant Staphylococcus aureus (VRSA) infection-induced oxidative stress in lymphocytes. VSSA and VRSA infections were developed in Swiss mice by intraperitoneal injection of 5 × 106 CFU/mL bacterial solutions. Nanoconjugated vancomycin was adminstrated to VSSA- and VRSA-infected mice at its effective dose for 10 days. Vancomycin was adminstrated to VSSA- and VRSA-infected mice at a similar dose, respectively, for 10 days. Vancomycin and nanoconjugated vancomycin were adminstrated to normal mice at their effective doses for 10 days. The result of this study reveals that in vivo VSSA and VRSA infection significantly increases the level of lipid peroxidation, protein oxidation, oxidized glutathione level, nitrite generation, nitrite release, and DNA damage and decreases the level of reduced glutathione, antioxidant enzyme status, and glutathione-dependent enzymes as compared to control group, which were increased or decreased significantly near to normal in nanoconjugated vancomycin-treated group. These findings suggest the potential use and beneficial role of nanoconjugated vancomycin against VSSA and VRSA infection-induced oxidative stress in lymphocytes.
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DOI：
10.1016/s2221-1691(11)60064-6
发表时间：
2011-02-01
影响因子：
1.7
作者：
Chakraborty, Subhankari Prasad;Mahapatra, Santanu Kar;Roy, Somenath
通讯作者：
Roy, Somenath
影响因子：
1.2
作者：
Li, Weihua;Tsubouchi, Ryoko;Yoshino, Masataka
通讯作者：
Yoshino, Masataka
影响因子：
--
作者：
Gautam N;Das S;Kar Mahapatra S;Chakraborty SP;Kundu PK;Roy S
通讯作者：
Roy S
影响因子：
3.1
作者：
Bayles, KW;Wesson, CA;Trumble, WR
通讯作者：
Trumble, WR
影响因子：
15.9
作者：
CHEUNG, AL;EBERHARDT, KJ;BAYER, AS
通讯作者：
BAYER, AS