Differential gene expression in abdomens of the malaria vector mosquito, Anopheles gambiae, after sugar feeding, blood feeding and Plasmodium berghei infection.
Differential gene expression in abdomens of the malaria vector mosquito, Anopheles gambiae, after sugar feeding, blood feeding and Plasmodium berghei infection.
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DOI：
10.1186/1471-2164-7-119
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发表时间：
2006-05-19
期刊：
影响因子：
4.4
通讯作者：
Collins FH
中科院分区：
文献类型：
--
作者：
Dana AN;Hillenmeyer ME;Lobo NF;Kern MK;Romans PA;Collins FH
cDNA 文库的大规模测序可以提供在特定的生物和环境条件下生物体中表达的基因的概况。我们分析了 3 个不同 cDNA 文库的 4541 个表达序列标签 (EST) 的序列，这些文库是从疟原虫感染易感的成年雌性冈比亚按蚊腹部创建的。这些文库是由糖喂养 (S)、大鼠血液喂养 (RB) 和伯氏疟原虫感染 (IRB) 的蚊子在吸血后 30 小时制成的，此时大多数寄生虫将转化动动子或非常早期的卵囊。 S、RB和IRB文库分别包含1727、1145和1669个高质量EST，平均长度为455个核苷酸(nt)。他们组装成 1975 个共有序列——567 个重叠群和 1408 个单序列。进行功能注释是为了注释基因产物的可能分子功能及其发挥作用的生物过程。对一个或多个文库中出现频率较高的基因进行数字Northern分析，并通过qRT-PCR验证5的表达结果。 1965 年 EST 中 13% 与冈比亚疟原虫基因组序列一致，代表新基因。这些与许多 EST 上存在的非翻译区 (UTR) 一起将为进一步的基因组注释提供信息。我们已经鉴定出 23 个可能参与调节细胞氧化环境的编码产物的基因和 25 个昆虫免疫基因。我们还鉴定出 25 个基因在喂食血液和/或喂食伯氏疟原虫感染的血液后相对于它们在喂食糖的雌性中的表达水平被上调或下调。
Large scale sequencing of cDNA libraries can provide profiles of genes expressed in an organism under defined biological and environmental circumstances. We have analyzed sequences of 4541 Expressed Sequence Tags (ESTs) from 3 different cDNA libraries created from abdomens from Plasmodium infection-susceptible adult female Anopheles gambiae. These libraries were made from sugar fed (S), rat blood fed (RB), and P. berghei-infected (IRB) mosquitoes at 30 hours after the blood meal, when most parasites would be transforming ookinetes or very early oocysts. The S, RB and IRB libraries contained 1727, 1145 and 1669 high quality ESTs, respectively, averaging 455 nucleotides (nt) in length. They assembled into 1975 consensus sequences – 567 contigs and 1408 singletons. Functional annotation was performed to annotate probable molecular functions of the gene products and the biological processes in which they function. Genes represented at high frequency in one or more of the libraries were subjected to digital Northern analysis and results on expression of 5 verified by qRT-PCR. 13% of the 1965 ESTs showing identity to the A. gambiae genome sequence represent novel genes. These, together with untranslated regions (UTR) present on many of the ESTs, will inform further genome annotation. We have identified 23 genes encoding products likely to be involved in regulating the cellular oxidative environment and 25 insect immunity genes. We also identified 25 genes as being up or down regulated following blood feeding and/or feeding with P. berghei infected blood relative to their expression levels in sugar fed females.
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影响因子：
4.4
作者：
Dana AN;Hong YS;Kern MK;Hillenmeyer ME;Harker BW;Lobo NF;Hogan JR;Romans P;Collins FH
通讯作者：
Collins FH
影响因子：
56.9
作者：
Christophides, GK;Zdobnov, E;Kafatos, FC
通讯作者：
Kafatos, FC
DOI：
10.1073/pnas.93.23.13066
发表时间：
1996-11-12
影响因子：
11.1
作者：
Dimopoulos, G;Richman, A;Louis, C
通讯作者：
Louis, C
影响因子：
4.8
作者：
Danielli, A;Kafatos, FC;Loukeris, TG
通讯作者：
Loukeris, TG
DOI：
10.1073/pnas.092274999
发表时间：
2002-06-25
影响因子：
11.1
作者：
Dimopoulos, G;Christophides, GK;Kafatos, FC
通讯作者：
Kafatos, FC