Sequence and expression analysis of rainbow trout CXCR2, CXCR3a and CXCR3b aids interpretation of lineage-specific conversion, loss and expansion of these receptors during vertebrate evolution.
Sequence and expression analysis of rainbow trout CXCR2, CXCR3a and CXCR3b aids interpretation of lineage-specific conversion, loss and expansion of these receptors during vertebrate evolution.
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虹鳟鱼 CXCR2、CXCR3a 和 CXCR3b 的序列和表达分析有助于解释脊椎动物进化过程中这些受体的谱系特异性转化、丢失和扩展
DOI：
10.1016/j.dci.2014.03.002
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发表时间：
2014-08
影响因子：
2.9
通讯作者：
Wang, Tiehui
中科院分区：
文献类型：
--
作者：
Xu, Qiaoqing;Li, Ronggai;Monte, Milena M.;Jiang, Yousheng;Nie, Pin;Holland, Jason W.;Secornbes, Chris J.;Wang, Tiehui
CXCR2、CXCR3a和CXCR3b的cDNA序列已在虹鲑鱼中被克隆。连锁的CXCR1/CXCR2和CXCR3a/CXCR3b基因座被认为存在于硬口动物的祖先中。CXCR1和CXCR2在哺乳动物中可能经历了基因转换，而CXCR3b已经丢失。与哺乳动物相比，鳍鱼具有更多的CXCR1-R3受体，但配体更少。鲑鱼CXCR1-R3在巨噬细胞和中性粒细胞中表达，CXCR1/R2也在B细胞中大量表达。趋化因子受体CXCR1-3与11种趋化因子(CXCL1-11)结合，这些趋化因子在哺乳动物中聚集在同一染色体上，但在鳍鱼中大部分缺失。第二个CXCR1/2，以及一个CXCR3a和CXCR3b基因已经在虹鲑鱼中被克隆。对叶鳍鱼、鳍鱼和四足类基因组中CXCR1-R3基因的分析表明，硬口鱼祖先可能同时含有CXCR1和CXCR2，以及CXCR3a和CXCR3b。在此基础上，将第一个鲑鱼CXCR1/2基因重新命名为CXCR1，并将新基因命名为CXCR2。CXCR1/R2基因座在鳍鱼中进一步扩大。关于CXCR3，哺乳动物似乎在进化过程中失去了CXCR3b，鸟类则同时失去了CXCR3a和CXCR3b。鲑鱼CXCR1-R3具有不同的组织表达模式，并受到PAMPs、促炎细胞因子和感染的差异调节。它们在巨噬细胞和中性粒细胞中高表达，CXCR1和CXCR2也在B细胞中表达。
The cDNA sequences of CXCR2, CXCR3a and CXCR3b have been cloned in rainbow trout. The linked CXCR1/CXCR2 and CXCR3a/CXCR3b loci are hypothesised to have been present in the teleostomian ancestor. CXCR1 and CXCR2 have likely undergone gene conversion whilst CXCR3b has been lost in mammals. Compared with mammals, ray-finned fish possess more CXCR1–R3 receptors, but fewer ligands. Trout CXCR1–R3 are expressed in macrophages and neutrophils, with CXCR1/R2 also abundant in B-cells. The chemokine receptors CXCR1–3 bind to 11 chemokines (CXCL1–11) that are clustered on the same chromosome in mammals but are largely missing in ray-finned fish. A second CXCR1/2, and a CXCR3a and CXCR3b gene have been cloned in rainbow trout. Analysis of CXCR1–R3 genes in lobe-finned fish, ray-finned fish and tetrapod genomes revealed that the teleostomian ancestor likely possessed loci containing both CXCR1 and CXCR2, and CXCR3a and CXCR3b. Based on this synteny analysis the first trout CXCR1/2 gene was renamed CXCR1, and the new gene CXCR2. The CXCR1/R2 locus was shown to have further expanded in ray-finned fish. In relation to CXCR3, mammals appear to have lost CXCR3b and birds both CXCR3a and CXCR3b during evolution. Trout CXCR1–R3 have distinct tissue expression patterns and are differentially modulated by PAMPs, proinflammatory cytokines and infections. They are highly expressed in macrophages and neutrophils, with CXCR1 and CXCR2 also expressed in B-cells.
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影响因子：
64.8
作者：
通讯作者：
--
影响因子：
4.4
作者：
Gorgoglione B;Wang T;Secombes CJ;Holland JW
通讯作者：
Holland JW
影响因子：
4.7
作者：
Ganassin, RC;Bols, NC
通讯作者：
Bols, NC
影响因子：
2.9
作者：
Huising, MO;Stolte, E;Verburg-van Kemenade, BML
通讯作者：
Verburg-van Kemenade, BML
影响因子：
2.7
作者：
Frydelund-Larsen, Lone;Penkowa, Milena;Pedersen, Bente Klarlund
通讯作者：
Pedersen, Bente Klarlund