Comparative genomics of bacteria in the genus Providencia isolated from wild Drosophila melanogaster.
Comparative genomics of bacteria in the genus Providencia isolated from wild Drosophila melanogaster.
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DOI：
10.1186/1471-2164-13-612
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发表时间：
2012-11-13
期刊：
影响因子：
4.4
通讯作者：
Lazzaro BP
中科院分区：
文献类型：
--
作者：
Galac MR;Lazzaro BP
比较基因组学可以是寻找细菌菌株和物种之间表型差异的遗传基础的第一步。从众多不同的环境中分离出了普罗维登西亚属的细菌。我们对P.rettgeri、P.snebia、P.alcalfaciens和P.burhodogranariea的基因组草案进行了测序、注释和比较。这些细菌最初都是作为野生黑腹果蝇的感染而分离出来的，之前已经被证明对实验感染的果蝇的毒力不同。我们发现，这些普罗维登西亚物种共享一个巨大的核心基因组，但也拥有不同的基因集，这些基因是每个分离株独一无二的。我们将这些分离物的基因组与从人类肠道分离的四个普罗维登西亚的基因组草稿进行了比较，发现核心基因组大小在纳入人类分离物后没有实质性的变化。我们在这些基因中发现了许多与黏附相关的基因，这些基因是每个基因组所特有的。我们还发现，每个分离物都至少有一个3型分泌系统(T3SS)，这是一个已知的毒力因子，尽管并不是所有鉴定的T3SS都属于同一个家族，也不是在同源基因组位置。这里研究的普罗维登西亚种的特点是基因组高度相似，这可能会延伸到该属内的其他物种和分离物。所有基因组中T3SS岛的存在表明，它们的存在不足以表明对黑腹果蝇的毒力，因为已知一些携带T3SS的分离物几乎不会造成死亡。黏附基因的变异和T3SS的存在表明宿主细胞黏附可能是普罗维登西亚毒力的一个重要方面。
Comparative genomics can be an initial step in finding the genetic basis for phenotypic differences among bacterial strains and species. Bacteria belonging to the genus Providencia have been isolated from numerous and varied environments. We sequenced, annotated and compared draft genomes of P. rettgeri, P. sneebia, P. alcalifaciens, and P. burhodogranariea. These bacterial species that were all originally isolated as infections of wild Drosophila melanogaster and have been previously shown to vary in virulence to experimentally infected flies. We found that these Providencia species share a large core genome, but also possess distinct sets of genes that are unique to each isolate. We compared the genomes of these isolates to draft genomes of four Providencia isolated from the human gut and found that the core genome size does not substantially change upon inclusion of the human isolates. We found many adhesion related genes among those genes that were unique to each genome. We also found that each isolate has at least one type 3 secretion system (T3SS), a known virulence factor, though not all identified T3SS belong to the same family nor are they in syntenic genomic locations. The Providencia species examined here are characterized by high degree of genomic similarity which will likely extend to other species and isolates within this genus. The presence of T3SS islands in all of the genomes reveal that their presence is not sufficient to indicate virulence towards D. melanogaster, since some of the T3SS-bearing isolates are known to cause little mortality. The variation in adhesion genes and the presence of T3SSs indicates that host cell adhesion is likely an important aspect of Providencia virulence.
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影响因子：
3.1
作者：
ALBERT, MJ;ALAM, K;MATHAN, MM
通讯作者：
MATHAN, MM
影响因子：
3
作者：
Interaminense, J. A.;Nascimento, D. C. O.;Lima-Filho, J. V.
通讯作者：
Lima-Filho, J. V.
DOI：
10.1111/j.1365-2672.1995.tb05022.x
发表时间：
1995-03-01
期刊：
JOURNAL OF APPLIED BACTERIOLOGY
影响因子：
--
作者：
JACKSON, TJ;WANG, HY;DOWDS, BCA
通讯作者：
DOWDS, BCA
影响因子：
3
作者：
ALBERT, MJ;ANSARUZZAMAN, M;FARUQUE, ASG
通讯作者：
FARUQUE, ASG
影响因子：
4.4
作者：
Corby-Harris, Vanessa;Pontaroli, Ana Clara;Promislow, Daniel E. L.
通讯作者：
Promislow, Daniel E. L.