Validation of the Friedewald Equation for Evaluation of Plasma LDL-Cholesterol.
Validation of the Friedewald Equation for Evaluation of Plasma LDL-Cholesterol.
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验证Friedewald方程以评估血浆LDL-胆固醇。
DOI：
10.3164/jcbn.2008036
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发表时间：
2008-07
影响因子：
2.4
通讯作者：
Tanaka E
中科院分区：
文献类型：
--
作者：
Fukuyama N;Homma K;Wakana N;Kudo K;Suyama A;Ohazama H;Tsuji C;Ishiwata K;Eguchi Y;Nakazawa H;Tanaka E
在大多数临床实验室，低密度脂蛋白（LDL）胆固醇通常是间接估计与弗里德瓦尔德方程或直接与氮测定。我们评估了通过两种方法获得的LDL-胆固醇值，以找到合适的禁食期，并评估最后一餐能量含量的影响。从28名健康志愿者中采集血液样本，这些志愿者食用标准餐（107 g碳水化合物，658 kcal），随后禁食12和18 h，或食用高能量餐（190 g碳水化合物，1011 kcal），禁食12 h。将禁食时间从12小时延长至18小时可降低血糖水平，但并不能降低三酰甘油、总胆固醇或高密度脂蛋白（HDL）胆固醇。用N-generous测定法测量的LDL-胆固醇水平没有变化（94.0 ± 21.5至96.3 ± 19.1 mg/dl）。空腹12 h（98.5 ± 21.4 mg/dl）和18 h（99.7 ± 20.2 mg/dl）后，采用弗里德瓦尔德方程计算的LDL-胆固醇水平也相似。高能量餐没有改变用N-geneous测定法测量的LDL-胆固醇水平（96.1 ± 21.2 mg/dl），或葡萄糖、三酰甘油、总胆固醇或HDL-胆固醇水平，但根据弗里德瓦尔德方程评估的LDL-胆固醇水平（92.6 ± 20.3 mg/dl）显著降低。空腹时间超过12小时是没有必要获得合理的血脂水平。弗里德瓦尔德方程得出的低密度脂蛋白-胆固醇水平高于N-异构测定的年轻日本女性谁吃了低能量的膳食，和较低的值时，他们吃了高能量的膳食。因此，在抽血之前，可能需要注意晚餐的能量。
In most clinical laboratories, low density lipoprotein (LDL) cholesterol is usually estimated indirectly with the Friedewald equation or directly with the N-geneous assay. We assessed LDL-cholesterol values obtained by both methods to find an appropriate fasting period and to assess the influence of the energy content of the last meal. Blood samples were taken from 28 healthy volunteers who had consumed a standard meal (107 g of carbohydrate, 658 kcal) followed by a fasting period of 12 and 18 h, or a high-energy meal (190 g of carbohydrate, 1011 kcal) with a fasting period of 12 h. Prolongation of the fasting period from 12 h to 18 h decreased glucose level, but did not decrease triacylglycerol, total cholesterol, or high density lipoprotein (HDL) cholesterol. LDL-cholesterol levels measured with the N-geneous assay did not change (94.0 ± 21.5 to 96.3 ± 19.1 mg/dl). LDL-cholesterol levels calculated with the Friedewald equation were also similar after fasting periods of 12 h (98.5 ± 21.4 mg/dl) and 18 h (99.7 ± 20.2 mg/dl). The high-energy meal did not change the level of LDL-cholesterol measured with the N-geneous assay (96.1 ± 21.2 mg/dl), or the glucose, triacylglycerol, total cholesterol, or HDL-cholesterol level, but LDL-cholesterol levels evaluated from the Friedewald equation (92.6 ± 20.3 mg/dl) became significantly lower. A fasting time longer than 12 h is not necessary to obtain reasonable blood lipid levels. The Friedewald equation gave higher LDL-cholesterol levels than N-geneous assay in young Japanese females who had eaten a low-energy meal, and lower values when they had eaten a high-energy meal. Thus, it may be necessary to pay attention to energy of nigh meal prior to blood withdrawal.
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影响因子：
2.4
作者：
A Ahmed K;Muniandy S;S Ismail I
通讯作者：
S Ismail I
影响因子：
2.8
作者：
LEVY, RI
通讯作者：
LEVY, RI
影响因子：
2.4
作者：
Ozsoy, Meral Bas;Pabuccuoglu, Aysun
通讯作者：
Pabuccuoglu, Aysun
影响因子：
--
作者：
GORDON, T;KANNEL, WB;DAWBER, TR
通讯作者：
DAWBER, TR
影响因子：
15.9
作者：
HAVEL, RJ;EDER, HA;BRAGDON, JH
通讯作者：
BRAGDON, JH