Integrin-targeting block copolymer probes for two-photon fluorescence bioimaging.
Integrin-targeting block copolymer probes for two-photon fluorescence bioimaging.
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DOI：
10.1021/bm1012212
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发表时间：
2011-02-14
影响因子：
6.2
通讯作者：
Belfield, Kevin D.
中科院分区：
文献类型：
--
作者：
Biswas, Sanchita;Wang, Xuhua;Morales, Alma R.;Ahn, Hyo-Yang;Belfield, Kevin D.
利用双光子荧光显微镜(2PFM)进行靶向分子成像是一种强大的化学生物学技术，并有可能用于许多疾病的非侵入性诊断和治疗。报道了一种基于降冰片烯的多功能嵌段共聚多功能支架的合成、光物理研究和生物成像。该支架含有生物相容性(PEG)、双光子荧光染料(荧基)和靶向(环-RGD肽)部分。这两种生物偶联物含有两种不同的荧基染料和共价连接在聚合物探针上的cRGDfK，在水中形成了球形胶束和自组装结构，并通过透射电子显微镜和差示扫描量热仪对其尺寸进行了分析。通过与新的探针孵育，以及使用α-7乳腺癌细胞的阴性对照研究和阻断实验，对高表达βv MCF3整合素的人上皮U87 MG细胞系的细胞活力和2PFM成像进行了研究。2PFM显微镜证实生物相容性探针在U87MF细胞的整合素富集区具有较高的选择性，而阻断实验和阴性对照MCF-7实验证实了新探针的整合素靶向能力。
Targeted molecular imaging with two-photon fluorescence microscopy (2PFM) is a powerful technique for chemical biology and, potentially, for non-invasive diagnosis and treatment of a number of diseases. The synthesis, photophysical studies, and bioimaging are reported for a versatile norbornene-based block copolymer multifunctional scaffold containing biocompatible (PEG), two-photon fluorescent dyes (fluorenyl), and targeting (cyclic-RGD peptide) moieties. The two bioconjugates, containing two different fluorenyl dyes and cRGDfK covalently attached to the polymer probe, formed a spherical micelle and self-assembled structure in water, for which size was analyzed by TEM and DLS. Cell-viability and 2PFM imaging of human epithelial U87MG cell lines that over express αvβ3 integrin was performed via incubation with the new probes, along with negative control studies using MCF-7 breast cancer cells and blocking experiments. 2PFM microscopy confirmed the high selectivity of the biocompatible probe in the integrin rich area in the U87MF cells while blocking as well as negative control MCF-7 experiments confirmed the integrin targeting ability of the new probes.
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影响因子：
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